Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment

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  • Henriette E. Autzen, University of California, San Francisco
  • ,
  • David Julius, University of California, San Francisco
  • ,
  • Yifan Cheng, University of California, San Francisco

Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubilization approaches for structural studies, including reconstitution into lipid nanoparticles. In this review, we discuss various approaches for preparing transmembrane proteins for structural determination with single-particle electron cryo microscopy (cryoEM).

Original languageEnglish
JournalCurrent Opinion in Structural Biology
Pages (from-to)259-268
Number of pages10
Publication statusPublished - Oct 2019

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