Measuring aggregates, self-association, and weak interactions in concentrated therapeutic antibody solutions

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  • Sumit K Chaturvedi, National Institutes of Health, Bethesda
  • ,
  • Arun Parupudi, AstraZeneca
  • ,
  • Kristian Juul-Madsen
  • Ai Nguyen, National Institutes of Health, Bethesda
  • ,
  • Thomas Vorup-Jensen
  • Sonia Dragulin-Otto, AstraZeneca
  • ,
  • Huaying Zhao, National Institutes of Health, Bethesda
  • ,
  • Reza Esfandiary, AstraZeneca
  • ,
  • Peter Schuck, National Institutes of Health, Bethesda

Monoclonal antibodies are a class of biotherapeutics used for an increasing variety of disorders, including cancer, autoimmune, neurodegenerative, and viral diseases. Besides their antigen specificity, therapeutic use also mandates control of their solution interactions and colloidal properties in order to achieve a stable, efficacious, non-immunogenic, and low viscosity antibody solution at concentrations in the range of 50-150 mg/mL. This requires characterization of their reversible self-association, aggregation, and weak attractive and repulsive interactions governing macromolecular distance distributions in solution. Simultaneous measurement of these properties, however, has been hampered by solution nonideality. Based on a recently introduced sedimentation velocity method for measuring macromolecular size distributions in a mean-field approximation for hydrodynamic interactions, we demonstrate simultaneous measurement of polydispersity and weak and strong solution interactions in a panel of antibodies with concentrations up to 45 mg/mL. By allowing approximately an order of magnitude higher concentrations than previously possible in sedimentation velocity size distribution analysis, this approach can substantially improve efficiency and sensitivity for characterizing polydispersity and interactions of therapeutic antibodies at or close to formulation conditions.

Original languageEnglish
Article number1810488
Number of pages13
Publication statusPublished - 2020

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