TY - JOUR
T1 - Measurements of cortical interstitium in biopsies from human kidney grafts
T2 - how representative and how reproducible?
AU - Ellingsen, Anne Ringer
AU - Nyengaard, Jens Randel
AU - Østerby, Ruth
AU - Jørgensen, Kaj Anker
AU - Petersen, Steffen Ellebaek
AU - Marcussen, Niels
PY - 2002/5
Y1 - 2002/5
N2 - BACKGROUND: The aim of the study was to evaluate how well a biopsy from one region of a human renal allograft represented biopsies from other regions regarding the renal interstitial tissue assessed by stereology. Furthermore, we wanted to evaluate the reproducibility of the measurements.METHODS: Punch biopsies (3 mm) from six regions in each kidney were obtained from seven explanted renal grafts with varying degrees of clinically diagnosed chronic and acute rejection. One kidney, removed for a minor pelvic tumour, served as reference material. Using point counting on PAS-stained sections, the volume fraction of the interstitial tissue per glomerular cortex V(V)(interstitium/cortex) was estimated. From each kidney, two of the six biopsies were re-evaluated by the same observer.RESULTS: V(V)(interstitium/cortex) varied from 0.25 to 0.78 between the explanted kidneys vs 0.26 in the reference kidney. Variations within the kidneys were low, expressed by standard deviations (SD) of between 0.04 and 0.06, and coefficients of variation (CV) between 0.06 and 0.22. The SD estimated from repeated measurements was 0.04 and CV was 0.07.CONCLUSIONS: Biopsies from one region of the kidney were found to be representative for estimates of interstitial tissue in explanted human kidney grafts, and the degree of reproducibility was high when using point counting, as in the present study.
AB - BACKGROUND: The aim of the study was to evaluate how well a biopsy from one region of a human renal allograft represented biopsies from other regions regarding the renal interstitial tissue assessed by stereology. Furthermore, we wanted to evaluate the reproducibility of the measurements.METHODS: Punch biopsies (3 mm) from six regions in each kidney were obtained from seven explanted renal grafts with varying degrees of clinically diagnosed chronic and acute rejection. One kidney, removed for a minor pelvic tumour, served as reference material. Using point counting on PAS-stained sections, the volume fraction of the interstitial tissue per glomerular cortex V(V)(interstitium/cortex) was estimated. From each kidney, two of the six biopsies were re-evaluated by the same observer.RESULTS: V(V)(interstitium/cortex) varied from 0.25 to 0.78 between the explanted kidneys vs 0.26 in the reference kidney. Variations within the kidneys were low, expressed by standard deviations (SD) of between 0.04 and 0.06, and coefficients of variation (CV) between 0.06 and 0.22. The SD estimated from repeated measurements was 0.04 and CV was 0.07.CONCLUSIONS: Biopsies from one region of the kidney were found to be representative for estimates of interstitial tissue in explanted human kidney grafts, and the degree of reproducibility was high when using point counting, as in the present study.
KW - Adult
KW - Aged
KW - Biopsy
KW - Female
KW - Humans
KW - Kidney/pathology
KW - Kidney Cortex/pathology
KW - Kidney Transplantation
KW - Male
KW - Middle Aged
KW - Reproducibility of Results
U2 - 10.1093/ndt/17.5.788
DO - 10.1093/ndt/17.5.788
M3 - Journal article
C2 - 11981064
SN - 0931-0509
VL - 17
SP - 788
EP - 792
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
IS - 5
ER -