Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Hossein Mohammad-Beigi; Yuya Hayashi; Christina Moeslund Zeuthen; Hoda Eskandari; Carsten Scavenius; Kristian Juul-Madsen; Thomas Vorup-Jensen; Jan J. Enghild; Duncan S Sutherland

doi:10.1038/s41467-020-18237-7

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Hossein Mohammad-Beigi, Yuya Hayashi, Christina Moeslund Zeuthen, Hoda Eskandari, Carsten Scavenius, Kristian Juul-Madsen, Thomas Vorup-Jensen, Jan J. Enghild, Duncan S Sutherland^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

149 Citations (Scopus)

Abstract

The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

Original language	English
Article number	4535
Journal	Nature Communications
Volume	11
Issue	1
Number of pages	16
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-020-18237-7
Publication status	Published - 1 Dec 2020

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title = "Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association",

abstract = "The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.",

author = "Hossein Mohammad-Beigi and Yuya Hayashi and Zeuthen, {Christina Moeslund} and Hoda Eskandari and Carsten Scavenius and Kristian Juul-Madsen and Thomas Vorup-Jensen and Enghild, {Jan J.} and Sutherland, {Duncan S}",

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doi = "10.1038/s41467-020-18237-7",

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Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association. / Mohammad-Beigi, Hossein; Hayashi, Yuya; Zeuthen, Christina Moeslund et al.
In: Nature Communications, Vol. 11, No. 1, 4535, 01.12.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

AU - Mohammad-Beigi, Hossein

AU - Hayashi, Yuya

AU - Zeuthen, Christina Moeslund

AU - Eskandari, Hoda

AU - Scavenius, Carsten

AU - Juul-Madsen, Kristian

AU - Vorup-Jensen, Thomas

AU - Enghild, Jan J.

AU - Sutherland, Duncan S

PY - 2020/12/1

Y1 - 2020/12/1

N2 - The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

AB - The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

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U2 - 10.1038/s41467-020-18237-7

DO - 10.1038/s41467-020-18237-7

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SN - 2041-1723

VL - 11

JO - Nature Communications

JF - Nature Communications

IS - 1

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ER -

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

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