Aarhus University Seal

Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H2O2) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO2-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO2-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H2O2. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO2-NSs showed directed locomotion in cell media with top speeds up to 50 μm s−1 when exposed to 300 mM H2O2 as a fuel, while reactors homogeneously coated with MnO2-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H2O2 from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.

Original languageEnglish
JournalJournal of Materials Chemistry B
Volume10
Issue24
Pages (from-to)4672-4683
Number of pages12
ISSN2050-750X
DOIs
Publication statusPublished - Jun 2022

Bibliographical note

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

See relations at Aarhus University Citationformats

ID: 277702178