Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells

Oksana K. Savchak, Nanying Wang, Miguel A. Ramos-Docampo, Paula de Dios Andres, Ana M. Sebastião, Filipa F. Ribeiro, Adam Armada-Moreira, Brigitte Städler*, Sandra H. Vaz*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H2O2) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO2-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO2-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H2O2. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO2-NSs showed directed locomotion in cell media with top speeds up to 50 μm s−1 when exposed to 300 mM H2O2 as a fuel, while reactors homogeneously coated with MnO2-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H2O2 from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.

Original languageEnglish
JournalJournal of Materials Chemistry B
Pages (from-to)4672-4683
Number of pages12
Publication statusPublished - Jun 2022


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