Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells

Oksana K. Savchak; Nanying Wang; Miguel A. Ramos-Docampo; Paula de Dios Andres; Ana M. Sebastião; Filipa F. Ribeiro; Adam Armada-Moreira; Brigitte Städler; Sandra H. Vaz

doi:10.1039/d2tb00393g

Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells

Oksana K. Savchak, Nanying Wang, Miguel A. Ramos-Docampo, Paula de Dios Andres, Ana M. Sebastião, Filipa F. Ribeiro, Adam Armada-Moreira, Brigitte Städler^*, Sandra H. Vaz^*

^*Corresponding author for this work

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

9 Citations (Scopus)

Abstract

Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H₂O₂) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO₂-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO₂-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H₂O₂. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO₂-NSs showed directed locomotion in cell media with top speeds up to 50 μm s⁻¹ when exposed to 300 mM H₂O₂ as a fuel, while reactors homogeneously coated with MnO₂-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H₂O₂ from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.

Original language	English
Journal	Journal of Materials Chemistry B
Volume	10
Issue	24
Pages (from-to)	4672-4683
Number of pages	12
ISSN	2050-750X
DOIs	https://doi.org/10.1039/d2tb00393g
Publication status	Published - Jun 2022

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title = "Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells",

abstract = "Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H2O2) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO2-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO2-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H2O2. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO2-NSs showed directed locomotion in cell media with top speeds up to 50 μm s−1 when exposed to 300 mM H2O2 as a fuel, while reactors homogeneously coated with MnO2-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H2O2 from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.",

author = "Savchak, {Oksana K.} and Nanying Wang and Ramos-Docampo, {Miguel A.} and {de Dios Andres}, Paula and Sebasti{\~a}o, {Ana M.} and Ribeiro, {Filipa F.} and Adam Armada-Moreira and Brigitte St{\"a}dler and Vaz, {Sandra H.}",

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year = "2022",

month = jun,

doi = "10.1039/d2tb00393g",

language = "English",

volume = "10",

pages = "4672--4683",

journal = "Journal of Materials Chemistry B",

issn = "2050-750X",

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Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells. / Savchak, Oksana K.; Wang, Nanying; Ramos-Docampo, Miguel A. et al.
In: Journal of Materials Chemistry B, Vol. 10, No. 24, 06.2022, p. 4672-4683.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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AU - Savchak, Oksana K.

AU - Wang, Nanying

AU - Ramos-Docampo, Miguel A.

AU - de Dios Andres, Paula

AU - Sebastião, Ana M.

AU - Ribeiro, Filipa F.

AU - Armada-Moreira, Adam

AU - Städler, Brigitte

AU - Vaz, Sandra H.

PY - 2022/6

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N2 - Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H2O2) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO2-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO2-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H2O2. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO2-NSs showed directed locomotion in cell media with top speeds up to 50 μm s−1 when exposed to 300 mM H2O2 as a fuel, while reactors homogeneously coated with MnO2-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H2O2 from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.

AB - Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H2O2) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO2-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO2-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H2O2. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO2-NSs showed directed locomotion in cell media with top speeds up to 50 μm s−1 when exposed to 300 mM H2O2 as a fuel, while reactors homogeneously coated with MnO2-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H2O2 from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.

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Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells

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