LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range.

Simona Radutoiu; Lene H Madsen; Esben B Madsen; Anna Jurkiewicz; Eigo Fukai; Esben M H Quistgaard; Anita S Albrektsen; Euan K James; Søren Thirup; Jens Stougaard

doi:10.1038/sj.emboj.7601826

LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range.

Simona Radutoiu, Lene H Madsen, Esben B Madsen, Anna Jurkiewicz, Eigo Fukai, Esben M H Quistgaard, Anita S Albrektsen, Euan K James, Søren Thirup, Jens Stougaard

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Legume-Rhizobium symbiosis is an example of selective cell recognition controlled by host/non-host determinants. Individual bacterial strains have a distinct host range enabling nodulation of a limited set of legume species and vice versa. We show here that expression of Lotus japonicus Nfr1 and Nfr5 Nod-factor receptor genes in Medicago truncatula and L. filicaulis, extends their host range to include bacterial strains, Mesorhizobium loti or DZL, normally infecting L. japonicus. As a result, the symbiotic program is induced, nodules develop and infection threads are formed. Using L. japonicus mutants and domain swaps between L. japonicus and L. filicaulis NFR1 and NFR5, we further demonstrate that LysM domains of the NFR1 and NFR5 receptors mediate perception of the bacterial Nod-factor signal and that recognition depends on the structure of the lipochitin-oligosaccharide Nod-factor. We show that a single amino-acid variation in the LysM2 domain of NFR5 changes recognition of the Nod-factor synthesized by the DZL strain and suggests a possible binding site for bacterial lipochitin-oligosaccharide signal molecules.
Udgivelsesdato: 2007-Sep-5

Original language	English
Journal	E M B O Journal
Volume	26
Issue	17
Pages (from-to)	3923-35
Number of pages	12
ISSN	0261-4189
DOIs	https://doi.org/10.1038/sj.emboj.7601826
Publication status	Published - 2007

Keywords

Alphaproteobacteria
Amino Acid Substitution
Bacterial Proteins
Chitin
Lipopolysaccharides
Lotus
Medicago truncatula
Models, Molecular
Mutation
Plant Proteins
Plant Roots
Protein Structure, Tertiary
Symbiosis

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10.1038/sj.emboj.7601826

Cite this

@article{8d55e720de1c11dc9e31000ea68e967b,

title = "LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range.",

abstract = "Legume-Rhizobium symbiosis is an example of selective cell recognition controlled by host/non-host determinants. Individual bacterial strains have a distinct host range enabling nodulation of a limited set of legume species and vice versa. We show here that expression of Lotus japonicus Nfr1 and Nfr5 Nod-factor receptor genes in Medicago truncatula and L. filicaulis, extends their host range to include bacterial strains, Mesorhizobium loti or DZL, normally infecting L. japonicus. As a result, the symbiotic program is induced, nodules develop and infection threads are formed. Using L. japonicus mutants and domain swaps between L. japonicus and L. filicaulis NFR1 and NFR5, we further demonstrate that LysM domains of the NFR1 and NFR5 receptors mediate perception of the bacterial Nod-factor signal and that recognition depends on the structure of the lipochitin-oligosaccharide Nod-factor. We show that a single amino-acid variation in the LysM2 domain of NFR5 changes recognition of the Nod-factor synthesized by the DZL strain and suggests a possible binding site for bacterial lipochitin-oligosaccharide signal molecules. Udgivelsesdato: 2007-Sep-5",

keywords = "Alphaproteobacteria, Amino Acid Substitution, Bacterial Proteins, Chitin, Lipopolysaccharides, Lotus, Medicago truncatula, Models, Molecular, Mutation, Plant Proteins, Plant Roots, Protein Structure, Tertiary, Symbiosis",

author = "Simona Radutoiu and Madsen, {Lene H} and Madsen, {Esben B} and Anna Jurkiewicz and Eigo Fukai and Quistgaard, {Esben M H} and Albrektsen, {Anita S} and James, {Euan K} and S{\o}ren Thirup and Jens Stougaard",

year = "2007",

doi = "10.1038/sj.emboj.7601826",

language = "English",

volume = "26",

pages = "3923--35",

journal = "E M B O Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell Publishing Ltd.",

number = "17",

}

LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range. / Radutoiu, Simona; Madsen, Lene H; Madsen, Esben B et al.
In: E M B O Journal, Vol. 26, No. 17, 2007, p. 3923-35.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - LysM domains mediate lipochitin-oligosaccharide recognition and Nfr genes extend the symbiotic host range.

AU - Radutoiu, Simona

AU - Madsen, Lene H

AU - Madsen, Esben B

AU - Jurkiewicz, Anna

AU - Fukai, Eigo

AU - Quistgaard, Esben M H

AU - Albrektsen, Anita S

AU - James, Euan K

AU - Thirup, Søren

AU - Stougaard, Jens

PY - 2007

Y1 - 2007

N2 - Legume-Rhizobium symbiosis is an example of selective cell recognition controlled by host/non-host determinants. Individual bacterial strains have a distinct host range enabling nodulation of a limited set of legume species and vice versa. We show here that expression of Lotus japonicus Nfr1 and Nfr5 Nod-factor receptor genes in Medicago truncatula and L. filicaulis, extends their host range to include bacterial strains, Mesorhizobium loti or DZL, normally infecting L. japonicus. As a result, the symbiotic program is induced, nodules develop and infection threads are formed. Using L. japonicus mutants and domain swaps between L. japonicus and L. filicaulis NFR1 and NFR5, we further demonstrate that LysM domains of the NFR1 and NFR5 receptors mediate perception of the bacterial Nod-factor signal and that recognition depends on the structure of the lipochitin-oligosaccharide Nod-factor. We show that a single amino-acid variation in the LysM2 domain of NFR5 changes recognition of the Nod-factor synthesized by the DZL strain and suggests a possible binding site for bacterial lipochitin-oligosaccharide signal molecules. Udgivelsesdato: 2007-Sep-5

AB - Legume-Rhizobium symbiosis is an example of selective cell recognition controlled by host/non-host determinants. Individual bacterial strains have a distinct host range enabling nodulation of a limited set of legume species and vice versa. We show here that expression of Lotus japonicus Nfr1 and Nfr5 Nod-factor receptor genes in Medicago truncatula and L. filicaulis, extends their host range to include bacterial strains, Mesorhizobium loti or DZL, normally infecting L. japonicus. As a result, the symbiotic program is induced, nodules develop and infection threads are formed. Using L. japonicus mutants and domain swaps between L. japonicus and L. filicaulis NFR1 and NFR5, we further demonstrate that LysM domains of the NFR1 and NFR5 receptors mediate perception of the bacterial Nod-factor signal and that recognition depends on the structure of the lipochitin-oligosaccharide Nod-factor. We show that a single amino-acid variation in the LysM2 domain of NFR5 changes recognition of the Nod-factor synthesized by the DZL strain and suggests a possible binding site for bacterial lipochitin-oligosaccharide signal molecules. Udgivelsesdato: 2007-Sep-5

KW - Alphaproteobacteria

KW - Amino Acid Substitution

KW - Bacterial Proteins

KW - Chitin

KW - Lipopolysaccharides

KW - Lotus

KW - Medicago truncatula

KW - Models, Molecular

KW - Mutation

KW - Plant Proteins

KW - Plant Roots

KW - Protein Structure, Tertiary

KW - Symbiosis

U2 - 10.1038/sj.emboj.7601826

DO - 10.1038/sj.emboj.7601826

M3 - Journal article

C2 - 17690687

SN - 0261-4189

VL - 26

SP - 3923

EP - 3935

JO - E M B O Journal

JF - E M B O Journal

IS - 17

ER -