Low-aspect ratio nanopatterns on bioinert alumina influence the response and morphology of osteoblast-like cells

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  • Isabel Wittenbrink, RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res
  • ,
  • Anne Hausmann, RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res, Denmark
  • Karolina Schickle, RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res, Denmark
  • Ines Lauria, RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res, Denmark
  • Roswitha Davtalab, RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res, Denmark
  • Morten Foss
  • Adrian Keller, Helmholtz Zentrum Dresden Rossendorf, Inst Ion Beam Phys & Mat Res, Univ Paderborn, Tech & Macromol Chem, Denmark
  • Horst Fischer, RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res, Denmark

Topographical features on the nanometer scale are known to influence cellular behavior. The response of specific cell types to various types of surface structures is currently still being investigated. Alumina ceramics play an important role as biomaterials, e.g., in medical and dental applications. In this study, we investigated the influence of nanoscale surface features with low aspect ratio (<0.1) on the response of osteoblast-like MG-63 cells. To this end, low-energy ion irradiation was employed to produce shallow nanoscale ripple patterns on Al2O3(0001) surfaces with lateral periodicities of 24 nm and 179 nm and heights of only 0.7 and 11.5 nm, respectively. The nanopatterning was found to increase the proliferation of MG-63 cells and may lead to pseudopodia alignment along the ripples. Furthermore, focal adhesion behavior and cell morphology were analyzed. We found that MG-63 cells are able to recognize surface nanopattems with extremely low vertical variations of less than 1 nm. In conclusion, it is shown that surface topography in the sub-nm range significantly influences the response of osteoblast-like cells. (c) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
JournalBiomaterials
Volume62
Pages (from-to)58-65
Number of pages8
ISSN0142-9612
DOIs
Publication statusPublished - 2015

    Research areas

  • Alumina, Nanotopography, Ion beam, Osteoblast, IN-VITRO, OSTEOGENIC DIFFERENTIATION, NANOPHASE CERAMICS, CONTACT GUIDANCE, SURFACES, ADHESION, PROLIFERATION, TOPOGRAPHY, ADSORPTION, DEPOSITION

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