Lotus japonicus SUNERGOS1 encodes a predicted subunit A of a DNA topoisomerase VI and is required for nodule differentiation and accommodation of rhizobial infection

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  • Hwi Joong Yoon, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Md Shakhawat Hossain, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Mark Held, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Hongwei Hou, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Marilyn Kehl, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Alexandre Tromas, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Shusei Sato, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan, Japan
  • Satoshi Tabata, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan, Japan
  • Stig Uggerhøj Andersen
  • Jens Stougaard
  • Loretta Ross, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada
  • Krzysztof Szczyglowski, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario, N5V 4T3, Canada; Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada., Canada

A symbiotic mutant of Lotus japonicus, called sunergos1-1 (suner1-1), originated from a har1-1 suppressor screen. suner1-1 supports epidermal infection by Mesorhizobium loti and initiates cell divisions for nodule primordia organogenesis. However, these processes appear to be temporarily stalled early during symbiotic interaction leading to a low nodule number phenotype. This defect is ephemeral and near wild-type nodule numbers are reached by suner1-1 at a later point after infection. Using an approach that combined map-based cloning and next-generation sequencing, we have identified the causative mutation and show that the suner1-1 phenotype is determined by a weak recessive allele, with the corresponding wild-type SUNER1 locus encoding a predicted subunit A of a DNA topoisomerase VI. Our data suggest that at least one function of SUNER1 during symbiosis is to participate in endoreduplication, which is an essential step during normal differentiation of functional, nitrogen-fixing nodules. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalPlant Journal
Volume78
Issue5
Pages (from-to)811-821
Number of pages10
ISSN0960-7412
DOIs
Publication statusPublished - Jun 2014

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