Lotus japonicus Genetic, Mutant, and Germplasm Resources

Masatsugu Hashiguchi; Hidenori Tanaka; Melody Muguerza; Ryo Akashi; Niels Nørgaard Sandal; Stig Uggerhøj Andersen; Shusei Sato

doi:10.1002/cppb.20070

Lotus japonicus Genetic, Mutant, and Germplasm Resources

Masatsugu Hashiguchi, Hidenori Tanaka, Melody Muguerza, Ryo Akashi, Niels Nørgaard Sandal, Stig Uggerhøj Andersen, Shusei Sato

Department of Molecular Biology and Genetics - Plant Molecular Biology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.

Original language	English
Article number	e20070
Journal	Current protocols in plant biology
Volume	3
Issue	2
Pages (from-to)	e20070
DOIs	https://doi.org/10.1002/cppb.20070
Publication status	Published - Jun 2018

Keywords

LORE1 lines
Lotus japonicus
genome information browser
legume
root culture system
wild accessions

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10.1002/cppb.20070

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title = "Lotus japonicus Genetic, Mutant, and Germplasm Resources",

abstract = "A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.",

keywords = "LORE1 lines, Lotus japonicus, genome information browser, legume, root culture system, wild accessions",

author = "Masatsugu Hashiguchi and Hidenori Tanaka and Melody Muguerza and Ryo Akashi and Sandal, {Niels N{\o}rgaard} and Andersen, {Stig Uggerh{\o}j} and Shusei Sato",

note = "Copyright {\textcopyright} 2018 John Wiley & Sons, Inc.",

year = "2018",

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AU - Sandal, Niels Nørgaard

AU - Andersen, Stig Uggerhøj

AU - Sato, Shusei

PY - 2018/6

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N2 - A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.

AB - A quarter of a century has passed since Lotus japonicus was proposed as a model legume because of its suitability for molecular genetic studies. Since then, a comprehensive set of genetic resources and tools has been developed, including recombinant inbred lines, a collection of wild accessions, published mutant lines, a large collection of mutant lines tagged with LORE1 insertions, cDNA clones with expressed sequence tag (EST) information, genomic clones with end-sequence information, and a reference genome sequence. Resource centers in Japan and Denmark ensure easy access to data and materials, and the resources have greatly facilitated L. japonicus research, thereby contributing to the molecular understanding of characteristic legume features such as endosymbiosis. Here, we provide detailed instructions for L. japonicus cultivation and describe how to order materials and access data using the resource center websites. The comprehensive overview presented here will make L. japonicus more easily accessible as a model system, especially for research groups new to L. japonicus research.

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KW - genome information browser

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KW - root culture system

KW - wild accessions

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Lotus japonicus Genetic, Mutant, and Germplasm Resources

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Keywords

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