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Loss of microRNA-targets in the 3'-untranslated region as a mechanism of retroviral insertional activation of Growth Factor Independence 1

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Loss of microRNA-targets in the 3'-untranslated region as a mechanism of retroviral insertional activation of Growth Factor Independence 1. / Dabrowska, Magdalena Julia; Dybkær, Karen; Johnsen, Hans Erik; Wang, Bruce; Wabl, Matthias; Pedersen, Finn Skou.

In: Journal of Virology, Vol. 83, No. 16, 2009, p. 8051-8061.

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Dabrowska, Magdalena Julia ; Dybkær, Karen ; Johnsen, Hans Erik ; Wang, Bruce ; Wabl, Matthias ; Pedersen, Finn Skou. / Loss of microRNA-targets in the 3'-untranslated region as a mechanism of retroviral insertional activation of Growth Factor Independence 1. In: Journal of Virology. 2009 ; Vol. 83, No. 16. pp. 8051-8061.

Bibtex

@article{e5a400505fea11deb2cc000ea68e967b,
title = "Loss of microRNA-targets in the 3'-untranslated region as a mechanism of retroviral insertional activation of Growth Factor Independence 1",
abstract = "The non-oncogene bearing retrovirus SL3-3 murine leukemia virus induces strictly T-cell lymphomas with a mean latency of 2-4 months in mice of the NMRI-inbred (i) strain. By high throughput sequencing of retroviral tags, we have identified the genomic region encoding the transcriptional repressor and oncogene, Growth Factor Independence 1 (Gfi1) as a frequent target for SL3-3 in the NMRI-i mouse genome. Twenty-four SL3-3 insertions were identified within a 1kb window of the 3'UTR of the Gfi1 gene - a clustering pattern unique for this lymphoma model. Expression analysis determined that the Gfi1 gene was transcriptionally activated by SL3-3 insertions, and an upregulation in Gfi1 protein expression was detected for tumors harboring insertions in the Gfi1 3'UTR. Here, we provide data in support of a mechanism by which retroviral insertions in the Gfi1 3'UTR decouple microRNA mediated posttranscriptional regulation.",
author = "Dabrowska, {Magdalena Julia} and Karen Dybk{\ae}r and Johnsen, {Hans Erik} and Bruce Wang and Matthias Wabl and Pedersen, {Finn Skou}",
year = "2009",
doi = "10.1128/JVI.00427-09",
language = "English",
volume = "83",
pages = "8051--8061",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "16",

}

RIS

TY - JOUR

T1 - Loss of microRNA-targets in the 3'-untranslated region as a mechanism of retroviral insertional activation of Growth Factor Independence 1

AU - Dabrowska, Magdalena Julia

AU - Dybkær, Karen

AU - Johnsen, Hans Erik

AU - Wang, Bruce

AU - Wabl, Matthias

AU - Pedersen, Finn Skou

PY - 2009

Y1 - 2009

N2 - The non-oncogene bearing retrovirus SL3-3 murine leukemia virus induces strictly T-cell lymphomas with a mean latency of 2-4 months in mice of the NMRI-inbred (i) strain. By high throughput sequencing of retroviral tags, we have identified the genomic region encoding the transcriptional repressor and oncogene, Growth Factor Independence 1 (Gfi1) as a frequent target for SL3-3 in the NMRI-i mouse genome. Twenty-four SL3-3 insertions were identified within a 1kb window of the 3'UTR of the Gfi1 gene - a clustering pattern unique for this lymphoma model. Expression analysis determined that the Gfi1 gene was transcriptionally activated by SL3-3 insertions, and an upregulation in Gfi1 protein expression was detected for tumors harboring insertions in the Gfi1 3'UTR. Here, we provide data in support of a mechanism by which retroviral insertions in the Gfi1 3'UTR decouple microRNA mediated posttranscriptional regulation.

AB - The non-oncogene bearing retrovirus SL3-3 murine leukemia virus induces strictly T-cell lymphomas with a mean latency of 2-4 months in mice of the NMRI-inbred (i) strain. By high throughput sequencing of retroviral tags, we have identified the genomic region encoding the transcriptional repressor and oncogene, Growth Factor Independence 1 (Gfi1) as a frequent target for SL3-3 in the NMRI-i mouse genome. Twenty-four SL3-3 insertions were identified within a 1kb window of the 3'UTR of the Gfi1 gene - a clustering pattern unique for this lymphoma model. Expression analysis determined that the Gfi1 gene was transcriptionally activated by SL3-3 insertions, and an upregulation in Gfi1 protein expression was detected for tumors harboring insertions in the Gfi1 3'UTR. Here, we provide data in support of a mechanism by which retroviral insertions in the Gfi1 3'UTR decouple microRNA mediated posttranscriptional regulation.

U2 - 10.1128/JVI.00427-09

DO - 10.1128/JVI.00427-09

M3 - Journal article

C2 - 19474094

VL - 83

SP - 8051

EP - 8061

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 16

ER -