Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy

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  • Ina Maria Schiessl
  • Katharina Fremter, Institute of Physiology, University of Regensburg, Regensburg, Germany; and.
  • ,
  • James L Burford, Department of Ophthalmology, University of Southern California, Los Angeles, CA, USA.
  • ,
  • Hayo Castrop, Institute of Physiology, University of Regensburg, Regensburg, Germany; and.
  • ,
  • Janos Peti-Peterdi, Department of Physiology and Neuroscience, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Intravital multiphoton microscopy of the kidney is a powerful technique to study alterations in tissue morphology and function simultaneously in the living animal and represents a dynamic and developing research tool in the field. Recent technological advances include serial intravital multiphoton microscopy of the same kidney regions over several weeks and combined with ex vivo histology for cellular biomarker expression of the same cells, which had been subject to serial imaging before. Thus, serial intravital multiphoton microscopy followed by ex vivo histology provides unique tools to perform long-term cell fate tracing of the same renal cells during physiological and pathophysiological conditions, thereby allowing the detection of structural changes of the same renal cells over time. Examples include renal cell migration and proliferation while linking these events to local functional alterations and eventually to the expression of distinct cellular biomarkers. Here, we provide a detailed step-by-step protocol to facilitate serial intravital multiphoton microscopy for long-term in vivo tracking of renal cells and subsequent ex vivo histology for immunohistological staining of the same cells in the fixed tissue.

Original languageEnglish
Title of host publicationImaging and Tracking Stem Cells : Methods and protocols
EditorsKursad Turksen
Number of pages20
Place of publicationNew York
PublisherHumana Press
Publication year2020
ISBN (print)978-1-0716-0626-1
ISBN (Electronic)978-1-0716-0627-8
Publication statusPublished - 2020
Externally publishedYes
SeriesMethods in Molecular Biology

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