Loading of polymyxin B onto anionic mesoporous silica nanoparticles retains antibacterial activity and enhances biocompatibility

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Loading of polymyxin B onto anionic mesoporous silica nanoparticles retains antibacterial activity and enhances biocompatibility. / Gounani, Zahra; Asadollahi, Mohammad A.; Meyer, Rikke L.; Arpanaei, Ayyoob.

In: International Journal of Pharmaceutics, Vol. 537, No. 1-2, 15.02.2018, p. 148-161.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Gounani, Z, Asadollahi, MA, Meyer, RL & Arpanaei, A 2018, 'Loading of polymyxin B onto anionic mesoporous silica nanoparticles retains antibacterial activity and enhances biocompatibility', International Journal of Pharmaceutics, vol. 537, no. 1-2, pp. 148-161. https://doi.org/10.1016/j.ijpharm.2017.12.039

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Author

Gounani, Zahra ; Asadollahi, Mohammad A. ; Meyer, Rikke L. ; Arpanaei, Ayyoob. / Loading of polymyxin B onto anionic mesoporous silica nanoparticles retains antibacterial activity and enhances biocompatibility. In: International Journal of Pharmaceutics. 2018 ; Vol. 537, No. 1-2. pp. 148-161.

Bibtex

@article{ffd5eb32d9544dc39c2e8745b849272d,
title = "Loading of polymyxin B onto anionic mesoporous silica nanoparticles retains antibacterial activity and enhances biocompatibility",
abstract = "Polymyxin B is a polycationic antibiotic used as the last line treatment against antibiotic-resistant Gram negative bacteria. However, application of polymyxin B is limited because of its toxicity effects. Herein, we used bare and surface modified mesoporous silica nanoparticles (MSNs) with an average diameter of 72.29 +/- 8.17 nm as adsorbent for polymyxin B to improve its therapeutic properties. The polymyxin B adsorption onto MSN surfaces was explained as a function of pH, type of buffer and surface charge of nanoparticles, according to the zeta-potential of silica nanoparticles and adsorption kinetics results. The highest value of the adsorption capacity (about 401 +/- 15.38 mg polymyxin B/g silica nanoparticles) was obtained for the bare nanoparticles in Tris buffer, pH 9. Release profiles of polymyxin B showed a sustained release pattern, fitting Power law and Hill models. The antibiotic molecules-loaded nanoparticles showed enhanced antibacterial activity compared to free antibiotic against different Gram negative bacteria. Biocompatibility evaluation results revealed that loading of polymyxin B onto MSNs can decrease the cytotoxicity effects of the drug by reducing ROS generation. Our results suggest that formulation of drugs by adsorption onto MSNs may offer a way forward to overcome the adverse effects of some antibiotics such as polymyxin B without compromising their antimicrobial properties.",
keywords = "Polymyxin B, Mesoporous silica nanoparticles, Adsorption, Antibiotic, Drug delivery, ALPHA-SYNUCLEIN FIBRILLATION, CONTROLLED DRUG-DELIVERY, PEPTIDE ADSORPTION, ANTIBIOTICS, RELEASE, SURFACE, FUNCTIONALIZATION, MECHANISMS, SIZE, INHIBITION",
author = "Zahra Gounani and Asadollahi, {Mohammad A.} and Meyer, {Rikke L.} and Ayyoob Arpanaei",
year = "2018",
month = "2",
day = "15",
doi = "10.1016/j.ijpharm.2017.12.039",
language = "English",
volume = "537",
pages = "148--161",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier BV",
number = "1-2",

}

RIS

TY - JOUR

T1 - Loading of polymyxin B onto anionic mesoporous silica nanoparticles retains antibacterial activity and enhances biocompatibility

AU - Gounani, Zahra

AU - Asadollahi, Mohammad A.

AU - Meyer, Rikke L.

AU - Arpanaei, Ayyoob

PY - 2018/2/15

Y1 - 2018/2/15

N2 - Polymyxin B is a polycationic antibiotic used as the last line treatment against antibiotic-resistant Gram negative bacteria. However, application of polymyxin B is limited because of its toxicity effects. Herein, we used bare and surface modified mesoporous silica nanoparticles (MSNs) with an average diameter of 72.29 +/- 8.17 nm as adsorbent for polymyxin B to improve its therapeutic properties. The polymyxin B adsorption onto MSN surfaces was explained as a function of pH, type of buffer and surface charge of nanoparticles, according to the zeta-potential of silica nanoparticles and adsorption kinetics results. The highest value of the adsorption capacity (about 401 +/- 15.38 mg polymyxin B/g silica nanoparticles) was obtained for the bare nanoparticles in Tris buffer, pH 9. Release profiles of polymyxin B showed a sustained release pattern, fitting Power law and Hill models. The antibiotic molecules-loaded nanoparticles showed enhanced antibacterial activity compared to free antibiotic against different Gram negative bacteria. Biocompatibility evaluation results revealed that loading of polymyxin B onto MSNs can decrease the cytotoxicity effects of the drug by reducing ROS generation. Our results suggest that formulation of drugs by adsorption onto MSNs may offer a way forward to overcome the adverse effects of some antibiotics such as polymyxin B without compromising their antimicrobial properties.

AB - Polymyxin B is a polycationic antibiotic used as the last line treatment against antibiotic-resistant Gram negative bacteria. However, application of polymyxin B is limited because of its toxicity effects. Herein, we used bare and surface modified mesoporous silica nanoparticles (MSNs) with an average diameter of 72.29 +/- 8.17 nm as adsorbent for polymyxin B to improve its therapeutic properties. The polymyxin B adsorption onto MSN surfaces was explained as a function of pH, type of buffer and surface charge of nanoparticles, according to the zeta-potential of silica nanoparticles and adsorption kinetics results. The highest value of the adsorption capacity (about 401 +/- 15.38 mg polymyxin B/g silica nanoparticles) was obtained for the bare nanoparticles in Tris buffer, pH 9. Release profiles of polymyxin B showed a sustained release pattern, fitting Power law and Hill models. The antibiotic molecules-loaded nanoparticles showed enhanced antibacterial activity compared to free antibiotic against different Gram negative bacteria. Biocompatibility evaluation results revealed that loading of polymyxin B onto MSNs can decrease the cytotoxicity effects of the drug by reducing ROS generation. Our results suggest that formulation of drugs by adsorption onto MSNs may offer a way forward to overcome the adverse effects of some antibiotics such as polymyxin B without compromising their antimicrobial properties.

KW - Polymyxin B

KW - Mesoporous silica nanoparticles

KW - Adsorption

KW - Antibiotic

KW - Drug delivery

KW - ALPHA-SYNUCLEIN FIBRILLATION

KW - CONTROLLED DRUG-DELIVERY

KW - PEPTIDE ADSORPTION

KW - ANTIBIOTICS

KW - RELEASE

KW - SURFACE

KW - FUNCTIONALIZATION

KW - MECHANISMS

KW - SIZE

KW - INHIBITION

U2 - 10.1016/j.ijpharm.2017.12.039

DO - 10.1016/j.ijpharm.2017.12.039

M3 - Journal article

C2 - 29278732

VL - 537

SP - 148

EP - 161

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -