Liquid chromatography-mass spectrometry method for the determination of polyethylene terephthalate and polybutylene terephthalate cyclic oligomers in blood samples

Dimitra Diamantidou, Orthodoxia Mastrogianni, Emmanouil Tsochatzis, Georgios Theodoridis, Nikolaos Raikos, Helen Gika, Stavros Kalogiannis*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Food contact materials (FCM) polyethylene terephthalate (PET) and polybutylene terephthalate (PBT) used extensively in food packaging may contain cyclic oligomers which may migrate into food and thus cause toxic effects on human health. A simple, fast, and sensitive ultra-high-performance liquid chromatography method quadrupole time-of-flight mass spectrometer was developed for the analysis of 7 cyclic oligomers in post-mortem blood samples. The targeted analytes were separated on a Waters BEH C18 (150 × 2.1 mm, 1.7 µm) analytical column by gradient elution. Calibration curves were constructed based on standard solutions and blood samples and Student’s t-test was applied to evaluate the matrix effect. The LODs ranged from 1.7 to 16.7 μg mL−1, while the method accuracy was assessed by recovery experiments and resulting within the range 84.2–114.6%. Such an analytical method for the determination of PET and PBT cyclic oligomers in biological samples is reported for the first time. The developed methodology allows the determination of these oligomers in blood providing a useful analytical tool to assess the exposure and thus the potential hazard and health risks associated with these non-intentionally added substances (NIAS) from PET and PBT FCM through food consumption. The method was validated and successfully applied to the analysis of 34 post-mortem whole blood samples. Polyethylene terephthalate trimer was detected in four of them, for the first time in literature. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
JournalAnalytical and Bioanalytical Chemistry
Volume414
Issue4
Pages (from-to)1503-1512
Number of pages10
ISSN1618-2642
DOIs
Publication statusPublished - Feb 2022

Keywords

  • Polybutylene terephthalate (PBT)
  • Polyethylene terephthalate (PET)
  • Quadrupole time-of-flight mass spectrometry (qTOF-MS)
  • Ultra high performance liquid chromatography (UHPLC)

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