Linking metabolites in eight bioactive forage species to their in vitro methane reduction potential across several cultivars and harvests

Supriya Verma*, Siegfried Wolffram, Juha-Pekka Salminen, Mario Hasler, Andreas Susenbeth, Ralf Blank, Friedhelm Taube, Christof Kluss, Carsten Stefan Malisch

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

An in vitro Hohenheim gas test was conducted to analyze the fermentation end-products from 17 cultivars of eight polyphenol containing forage species. The polyphenol composition and proanthocyanidin (PA) structural features of all the cultivars were analyzed with UPLC-MS/MS in leaves of vegetative or generative plants. The samples were incubated with and without polyethylene glycol (PEG, a tannin-binding agent) to separate the tannin-effect on methane (CH4, ml/200 mg DM) production from that of forage quality. Sulla and big trefoil, two particularly PA rich species, were found to have the highest CH4 reduction potential of up to 47% when compared to the samples without PEG. However, concomitant reduction in gas production (GP, ml/200 mg DM) of up to 44% was also observed. An increase in both GP and CH4 production under PEG treatments, confirms the role of tannins in CH4 reduction. Moreover, PA structural features and concentration were found to be an important source of variation for CH4 production from PA containing species. Despite having low polyphenol concentrations, chicory and plantain were found to reduce CH4 production without reducing GP. Additionally, interspecies variability was found to be higher than intraspecies variability, and these results were consistent across growth stages, indicating the findings' representativeness.

Original languageEnglish
Article number10454
JournalScientific Reports
Volume12
Issue1
Number of pages15
ISSN2045-2322
DOIs
Publication statusPublished - 21 Jun 2022

Keywords

  • NEUTRAL DETERGENT FIBER
  • CONDENSED TANNINS
  • RUMEN FERMENTATION
  • NUTRITIVE-VALUE
  • DIET DIGESTIBILITY
  • RUMINAL METHANE
  • EMISSION
  • CHICORY
  • PERFORMANCE
  • MITIGATION

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