Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain

Esben M Quistgaard; Peder Madsen; Morten K Grøftehauge; Poul Nissen; Claus M Petersen; Søren S Thirup

doi:10.1038/nsmb.1543

Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain

Esben M Quistgaard, Peder Madsen, Morten K Grøftehauge, Poul Nissen, Claus M Petersen, Søren S Thirup

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

132 Citations (Scopus)

Abstract

The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of nerve growth factor-beta, are present in the tunnel and that competition for binding relates to the restricted space inside the propeller.

Original language	English
Journal	Nature Structural and Molecular Biology
Volume	16
Issue	1
Pages (from-to)	96-8
Number of pages	3
ISSN	1545-9993
DOIs	https://doi.org/10.1038/nsmb.1543
Publication status	Published - 2009

Keywords

Adaptor Proteins, Vesicular Transport
Animals
Apoptosis
Binding Sites
Ligands
Mammals
Models, Molecular
Neurons
Neurotensin
Protein Binding
Protein Conformation

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title = "Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain",

abstract = "The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of nerve growth factor-beta, are present in the tunnel and that competition for binding relates to the restricted space inside the propeller.",

keywords = "Adaptor Proteins, Vesicular Transport, Animals, Apoptosis, Binding Sites, Ligands, Mammals, Models, Molecular, Neurons, Neurotensin, Protein Binding, Protein Conformation",

author = "Quistgaard, {Esben M} and Peder Madsen and Gr{\o}ftehauge, {Morten K} and Poul Nissen and Petersen, {Claus M} and Thirup, {S{\o}ren S}",

year = "2009",

doi = "10.1038/nsmb.1543",

language = "English",

volume = "16",

pages = "96--8",

journal = "Nature Structural and Molecular Biology",

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Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain. / Quistgaard, Esben M; Madsen, Peder; Grøftehauge, Morten K et al.
In: Nature Structural and Molecular Biology, Vol. 16, No. 1, 2009, p. 96-8.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain

AU - Quistgaard, Esben M

AU - Madsen, Peder

AU - Grøftehauge, Morten K

AU - Nissen, Poul

AU - Petersen, Claus M

AU - Thirup, Søren S

PY - 2009

Y1 - 2009

N2 - The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of nerve growth factor-beta, are present in the tunnel and that competition for binding relates to the restricted space inside the propeller.

AB - The structure of the Sortilin ectodomain in complex with neurotensin has been determined at 2-A resolution, revealing that the C-terminal part of neurotensin binds in the tunnel of a ten-bladed beta-propeller domain. Binding competition studies suggest that additional binding sites, for example, for the prodomain of nerve growth factor-beta, are present in the tunnel and that competition for binding relates to the restricted space inside the propeller.

KW - Adaptor Proteins, Vesicular Transport

KW - Animals

KW - Apoptosis

KW - Binding Sites

KW - Ligands

KW - Mammals

KW - Models, Molecular

KW - Neurons

KW - Neurotensin

KW - Protein Binding

KW - Protein Conformation

U2 - 10.1038/nsmb.1543

DO - 10.1038/nsmb.1543

M3 - Journal article

C2 - 19122660

SN - 1545-9993

VL - 16

SP - 96

EP - 98

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

IS - 1

ER -

Ligands bind to Sortilin in the tunnel of a ten-bladed beta-propeller domain

Abstract

Keywords

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