Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Zoltan Bozsoki; Kira Gysel; Simon B. Hansen; Damiano Lironi; Christina Krönauer; Feng Feng; Noor de Jong; Maria Vinther; Manoj Kamble; Mikkel B. Thygesen; Ebbe Engholm; Christian Kofoed; Sébastien Fort; John T. Sullivan; Clive W. Ronson; Knud J. Jensen; Mickaël Blaise; Giles Oldroyd; Jens Stougaard; Kasper R. Andersen; Simona Radutoiu

doi:10.1126/science.abb3377

Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

Zoltan Bozsoki, Kira Gysel, Simon B. Hansen, Damiano Lironi, Christina Krönauer, Feng Feng, Noor de Jong, Maria Vinther, Manoj Kamble, Mikkel B. Thygesen, Ebbe Engholm, Christian Kofoed, Sébastien Fort, John T. Sullivan, Clive W. Ronson, Knud J. Jensen, Mickaël Blaise, Giles Oldroyd, Jens Stougaard, Kasper R. Andersen^*Simona Radutoiu^*

^*Corresponding author for this work

Department of Molecular Biology and Genetics - Plant Molecular Biology

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

103 Citations (Scopus)

Abstract

Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicus initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.

Original language	English
Journal	Science (New York, N.Y.)
Volume	369
Issue	6504
Pages (from-to)	663-670
Number of pages	8
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.abb3377
Publication status	Published - Aug 2020

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Bozsoki, Z., Gysel, K., Hansen, S. B., Lironi, D., Krönauer, C., Feng, F., de Jong, N., Vinther, M., Kamble, M., Thygesen, M. B., Engholm, E., Kofoed, C., Fort, S., Sullivan, J. T., Ronson, C. W., Jensen, K. J., Blaise, M., Oldroyd, G., Stougaard, J., ... Radutoiu, S. (2020). Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity. Science (New York, N.Y.), 369(6504), 663-670. https://doi.org/10.1126/science.abb3377

@article{9148c838bfd4481a89d684bf5b0452ac,

title = "Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity",

abstract = "Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicus initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.",

author = "Zoltan Bozsoki and Kira Gysel and Hansen, {Simon B.} and Damiano Lironi and Christina Kr{\"o}nauer and Feng Feng and {de Jong}, Noor and Maria Vinther and Manoj Kamble and Thygesen, {Mikkel B.} and Ebbe Engholm and Christian Kofoed and S{\'e}bastien Fort and Sullivan, {John T.} and Ronson, {Clive W.} and Jensen, {Knud J.} and Micka{\"e}l Blaise and Giles Oldroyd and Jens Stougaard and Andersen, {Kasper R.} and Simona Radutoiu",

year = "2020",

month = aug,

doi = "10.1126/science.abb3377",

language = "English",

volume = "369",

pages = "663--670",

journal = "Science (New York, N.Y.)",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6504",

}

Bozsoki, Z, Gysel, K, Hansen, SB, Lironi, D, Krönauer, C, Feng, F, de Jong, N , Vinther, M, Kamble, M, Thygesen, MB, Engholm, E, Kofoed, C, Fort, S, Sullivan, JT, Ronson, CW, Jensen, KJ, Blaise, M, Oldroyd, G, Stougaard, J , Andersen, KR & Radutoiu, S 2020, 'Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity', Science (New York, N.Y.), vol. 369, no. 6504, pp. 663-670. https://doi.org/10.1126/science.abb3377

TY - JOUR

T1 - Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

AU - Bozsoki, Zoltan

AU - Gysel, Kira

AU - Hansen, Simon B.

AU - Lironi, Damiano

AU - Krönauer, Christina

AU - Feng, Feng

AU - de Jong, Noor

AU - Vinther, Maria

AU - Kamble, Manoj

AU - Thygesen, Mikkel B.

AU - Engholm, Ebbe

AU - Kofoed, Christian

AU - Fort, Sébastien

AU - Sullivan, John T.

AU - Ronson, Clive W.

AU - Jensen, Knud J.

AU - Blaise, Mickaël

AU - Oldroyd, Giles

AU - Stougaard, Jens

AU - Andersen, Kasper R.

AU - Radutoiu, Simona

PY - 2020/8

Y1 - 2020/8

N2 - Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicus initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.

AB - Plants evolved lysine motif (LysM) receptors to recognize and parse microbial elicitors and drive intracellular signaling to limit or facilitate microbial colonization. We investigated how chitin and nodulation (Nod) factor receptors of Lotus japonicus initiate differential signaling of immunity or root nodule symbiosis. Two motifs in the LysM1 domains of these receptors determine specific recognition of ligands and discriminate between their in planta functions. These motifs define the ligand-binding site and make up the most structurally divergent regions in cognate Nod factor receptors. An adjacent motif modulates the specificity for Nod factor recognition and determines the selection of compatible rhizobial symbionts in legumes. We also identified how binding specificities in LysM receptors can be altered to facilitate Nod factor recognition and signaling from a chitin receptor, advancing the prospects of engineering rhizobial symbiosis into nonlegumes.

UR - http://www.scopus.com/inward/record.url?scp=85089301819&partnerID=8YFLogxK

U2 - 10.1126/science.abb3377

DO - 10.1126/science.abb3377

M3 - Journal article

C2 - 32764065

AN - SCOPUS:85089301819

SN - 0036-8075

VL - 369

SP - 663

EP - 670

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 6504

ER -

Ligand-recognizing motifs in plant LysM receptors are major determinants of specificity

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