Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation.

Lotte Ebdrup; Jan Krog; Asger Granfeldt; Pernille Ø Larsen; Christian Vestergaard; Marianne Hokland; Else Tønnesen

doi:10.1111/j.1600-0463.2008.00982.x

Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation.

Lotte Ebdrup, Jan Krog, Asger Granfeldt, Pernille Ø Larsen, Christian Vestergaard, Marianne Hokland, Else Tønnesen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Systemic administered lipopolysaccharide (LPS) induces a cytokine response in peripheral blood without correlations with cytokine content at the organ level. We hypothesised (1) that cytokine mRNA expression in peripheral blood mononuclear cells (PBMCs) preceded the plasma cytokine increase during endotoxaemia and (2) that statins as anti-inflammatory agents modified the LPS-induced cytokine responses. 30 pigs were randomised into 3 groups: placebo (I) or atorvastatin 80 mg (II) for 21 days, followed by LPS-infusion on day 22, or controls (III). LPS was infused at increasing concentrations (2.5 to 15 mug/kg/h) for 30 min, followed by sustained infusion (2.5 mug/kg/h) for 330 min. We measured plasma IL-6, IL-10, and TNF-alpha, and their mRNA expression in PBMCs during the LPS-infusion, and the cytokine content in kidney and heart biopsies at 360 min. LPS reduced TNF-alpha mRNA in PBMCs at 60 min, whereas IL-6 mRNA increased at 240 min. There were no correlations with plasma cytokines, which peaked at 60 min (IL-10 and TNF-alpha) and 240 min (IL-6). Cytokine content did not increase in organs, and no effects of statins could be demonstrated. In conclusion, LPS-infusion reduced leukocyte TNF-alpha mRNA and increased IL-6 mRNA, whereas plasma TNF-alpha, IL-6, and IL-10 increased markedly.

Original language	English
Journal	Acta Pathologica Microbiologica et Immunologica Scandinavica
Volume	116
Issue	5
Pages (from-to)	352-60
Number of pages	8
ISSN	0903-4641
DOIs	https://doi.org/10.1111/j.1600-0463.2008.00982.x
Publication status	Published - 2008

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10.1111/j.1600-0463.2008.00982.x

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title = "Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation.",

abstract = "Systemic administered lipopolysaccharide (LPS) induces a cytokine response in peripheral blood without correlations with cytokine content at the organ level. We hypothesised (1) that cytokine mRNA expression in peripheral blood mononuclear cells (PBMCs) preceded the plasma cytokine increase during endotoxaemia and (2) that statins as anti-inflammatory agents modified the LPS-induced cytokine responses. 30 pigs were randomised into 3 groups: placebo (I) or atorvastatin 80 mg (II) for 21 days, followed by LPS-infusion on day 22, or controls (III). LPS was infused at increasing concentrations (2.5 to 15 mug/kg/h) for 30 min, followed by sustained infusion (2.5 mug/kg/h) for 330 min. We measured plasma IL-6, IL-10, and TNF-alpha, and their mRNA expression in PBMCs during the LPS-infusion, and the cytokine content in kidney and heart biopsies at 360 min. LPS reduced TNF-alpha mRNA in PBMCs at 60 min, whereas IL-6 mRNA increased at 240 min. There were no correlations with plasma cytokines, which peaked at 60 min (IL-10 and TNF-alpha) and 240 min (IL-6). Cytokine content did not increase in organs, and no effects of statins could be demonstrated. In conclusion, LPS-infusion reduced leukocyte TNF-alpha mRNA and increased IL-6 mRNA, whereas plasma TNF-alpha, IL-6, and IL-10 increased markedly.",

author = "Lotte Ebdrup and Jan Krog and Asger Granfeldt and Larsen, {Pernille {\O}} and Christian Vestergaard and Marianne Hokland and Else T{\o}nnesen",

year = "2008",

doi = "10.1111/j.1600-0463.2008.00982.x",

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Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation. / Ebdrup, Lotte; Krog, Jan ; Granfeldt, Asger et al.
In: Acta Pathologica Microbiologica et Immunologica Scandinavica, Vol. 116, No. 5, 2008, p. 352-60.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation.

AU - Ebdrup, Lotte

AU - Krog, Jan

AU - Granfeldt, Asger

AU - Larsen, Pernille Ø

AU - Vestergaard, Christian

AU - Hokland, Marianne

AU - Tønnesen, Else

PY - 2008

Y1 - 2008

N2 - Systemic administered lipopolysaccharide (LPS) induces a cytokine response in peripheral blood without correlations with cytokine content at the organ level. We hypothesised (1) that cytokine mRNA expression in peripheral blood mononuclear cells (PBMCs) preceded the plasma cytokine increase during endotoxaemia and (2) that statins as anti-inflammatory agents modified the LPS-induced cytokine responses. 30 pigs were randomised into 3 groups: placebo (I) or atorvastatin 80 mg (II) for 21 days, followed by LPS-infusion on day 22, or controls (III). LPS was infused at increasing concentrations (2.5 to 15 mug/kg/h) for 30 min, followed by sustained infusion (2.5 mug/kg/h) for 330 min. We measured plasma IL-6, IL-10, and TNF-alpha, and their mRNA expression in PBMCs during the LPS-infusion, and the cytokine content in kidney and heart biopsies at 360 min. LPS reduced TNF-alpha mRNA in PBMCs at 60 min, whereas IL-6 mRNA increased at 240 min. There were no correlations with plasma cytokines, which peaked at 60 min (IL-10 and TNF-alpha) and 240 min (IL-6). Cytokine content did not increase in organs, and no effects of statins could be demonstrated. In conclusion, LPS-infusion reduced leukocyte TNF-alpha mRNA and increased IL-6 mRNA, whereas plasma TNF-alpha, IL-6, and IL-10 increased markedly.

AB - Systemic administered lipopolysaccharide (LPS) induces a cytokine response in peripheral blood without correlations with cytokine content at the organ level. We hypothesised (1) that cytokine mRNA expression in peripheral blood mononuclear cells (PBMCs) preceded the plasma cytokine increase during endotoxaemia and (2) that statins as anti-inflammatory agents modified the LPS-induced cytokine responses. 30 pigs were randomised into 3 groups: placebo (I) or atorvastatin 80 mg (II) for 21 days, followed by LPS-infusion on day 22, or controls (III). LPS was infused at increasing concentrations (2.5 to 15 mug/kg/h) for 30 min, followed by sustained infusion (2.5 mug/kg/h) for 330 min. We measured plasma IL-6, IL-10, and TNF-alpha, and their mRNA expression in PBMCs during the LPS-infusion, and the cytokine content in kidney and heart biopsies at 360 min. LPS reduced TNF-alpha mRNA in PBMCs at 60 min, whereas IL-6 mRNA increased at 240 min. There were no correlations with plasma cytokines, which peaked at 60 min (IL-10 and TNF-alpha) and 240 min (IL-6). Cytokine content did not increase in organs, and no effects of statins could be demonstrated. In conclusion, LPS-infusion reduced leukocyte TNF-alpha mRNA and increased IL-6 mRNA, whereas plasma TNF-alpha, IL-6, and IL-10 increased markedly.

U2 - 10.1111/j.1600-0463.2008.00982.x

DO - 10.1111/j.1600-0463.2008.00982.x

M3 - Journal article

C2 - 18452425

SN - 0903-4641

VL - 116

SP - 352

EP - 360

JO - Acta Pathologica Microbiologica et Immunologica Scandinavica

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica

IS - 5

ER -

Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation.

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