Legume anchor markers link syntenic regions between Phaseolus vulgaris, Lotus japonicus, Medicago truncatula and Arachis

TY - JOUR

T1 - Legume anchor markers link syntenic regions between Phaseolus vulgaris, Lotus japonicus, Medicago truncatula and Arachis

AU - Hougaard, Birgit Kristine

AU - Madsen, Lene Heegaard

AU - Sandal, Niels

AU - de Carvalho Moretzsohn, Marcio

AU - Fredslund, Jakob

AU - Schauser, Leif

AU - Nielsen, Anna Marie

AU - Rohde, Trine

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Bertioli, David John

AU - Stougaard, Jens

PY - 2008

Y1 - 2008

N2 - We have previously described a bioinformatics pipeline identifying comparative anchor-tagged sequence (CATS) loci, combined with design of intron-spanning primers. The derived anchor markers defining the linkage position of homologous genes are essential for evaluating genome conservation among related species and facilitate transfer of genetic and genome information between species. Here we validate this global approach in the common bean and in the AA genome complement of the allotetraploid peanut. We present the successful conversion of approximately 50% of the bioinformatics-defined primers into legume anchor markers in bean and diploid Arachis species. One hundred and four new loci representing single-copy genes were added to the existing bean map. These new legume anchor-marker loci enabled the alignment of genetic linkage maps through corresponding genes and provided an estimate of the extent of synteny and collinearity. Extensive macrosynteny between Lotus and bean was uncovered on 8 of the 11 bean chromosomes and large blocks of macrosynteny were also found between bean and Medicago. This suggests that anchor markers can facilitate a better understanding of the genes and genetics of important traits in crops with largely uncharacterized genomes using genetic and genome information from related model plants.

AB - We have previously described a bioinformatics pipeline identifying comparative anchor-tagged sequence (CATS) loci, combined with design of intron-spanning primers. The derived anchor markers defining the linkage position of homologous genes are essential for evaluating genome conservation among related species and facilitate transfer of genetic and genome information between species. Here we validate this global approach in the common bean and in the AA genome complement of the allotetraploid peanut. We present the successful conversion of approximately 50% of the bioinformatics-defined primers into legume anchor markers in bean and diploid Arachis species. One hundred and four new loci representing single-copy genes were added to the existing bean map. These new legume anchor-marker loci enabled the alignment of genetic linkage maps through corresponding genes and provided an estimate of the extent of synteny and collinearity. Extensive macrosynteny between Lotus and bean was uncovered on 8 of the 11 bean chromosomes and large blocks of macrosynteny were also found between bean and Medicago. This suggests that anchor markers can facilitate a better understanding of the genes and genetics of important traits in crops with largely uncharacterized genomes using genetic and genome information from related model plants.

KW - Arachis hypogaea

KW - Genetic Markers

KW - Genome, Plant

KW - Linkage (Genetics)

KW - Lotus

KW - Medicago truncatula

KW - Phaseolus

KW - Synteny

U2 - 10.1534/genetics.108.090084

DO - 10.1534/genetics.108.090084

M3 - Journal article

C2 - 18689902

SN - 0016-6731

VL - 179

SP - 2299

EP - 2312

JO - Genetics

JF - Genetics

IS - 4

ER -