Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53

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Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53. / Guan, Xiao lu; Zhang, Bao cun; Sun, Li.

In: Developmental and Comparative Immunology, Vol. 103, 103531, 02.2020.

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Guan, Xiao lu ; Zhang, Bao cun ; Sun, Li. / Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53. In: Developmental and Comparative Immunology. 2020 ; Vol. 103.

Bibtex

@article{17dd02b2dcbc40db8c75386ffe4cc688,
title = "Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53",
abstract = "MicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3′UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda.",
keywords = "Autophagy, Bacterial infection, Immune defense, miRNA, Paralichthys olivaceus",
author = "Guan, {Xiao lu} and Zhang, {Bao cun} and Li Sun",
year = "2020",
month = feb,
doi = "10.1016/j.dci.2019.103531",
language = "English",
volume = "103",
journal = "Developmental & Comparative Immunology",
issn = "0145-305X",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53

AU - Guan, Xiao lu

AU - Zhang, Bao cun

AU - Sun, Li

PY - 2020/2

Y1 - 2020/2

N2 - MicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3′UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda.

AB - MicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3′UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda.

KW - Autophagy

KW - Bacterial infection

KW - Immune defense

KW - miRNA

KW - Paralichthys olivaceus

UR - http://www.scopus.com/inward/record.url?scp=85074177686&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2019.103531

DO - 10.1016/j.dci.2019.103531

M3 - Journal article

C2 - 31668931

AN - SCOPUS:85074177686

VL - 103

JO - Developmental & Comparative Immunology

JF - Developmental & Comparative Immunology

SN - 0145-305X

M1 - 103531

ER -