Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53

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  • Xiao lu Guan, Institute of Oceanology Chinese Academy of Sciences, Qingdao National Laboratory for Marine Science and Technology
  • ,
  • Bao cun Zhang
  • Li Sun, Institute of Oceanology Chinese Academy of Sciences, Qingdao National Laboratory for Marine Science and Technology

MicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3′UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda.

Original languageEnglish
Article number103531
JournalDevelopmental and Comparative Immunology
Volume103
ISSN0145-305X
DOIs
Publication statusPublished - Feb 2020

    Research areas

  • Autophagy, Bacterial infection, Immune defense, miRNA, Paralichthys olivaceus

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