Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample

C. Tesauro; B. Ferrando; X. Ma; M. L. Jepsen; Anne Kathrine Ivarsen; R. Frohlich; T. Stevnsner; B. R. Knudsen; Y. P. Ho

doi:10.1039/c7ra03384b

Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample

C. Tesauro, B. Ferrando, X. Ma, M. L. Jepsen, Anne Kathrine Ivarsen, R. Frohlich, T. Stevnsner, B. R. Knudsen, Y. P. Ho^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

10 Citations (Scopus)

226 Downloads (Pure)

Abstract

Isolation of subcellular fractions from a limited amount of clinical sample is imperative for the continuous advancement of biological and clinical research. For example, analyses of pure and functional mitochondria from patient samples are expected not only to expand our knowledge towards the basic biological mechanisms of mitochondrial function, but also to elucidate how mitochondria are involved in the development of diseases such as cancers, premature aging syndromes, diabetes and neurodegenerative disorders. While currently available methods are mostly laborious and not suitable for small-scale analyses, we present a novel and facile approach to isolate mitochondria from limited amounts of biological samples by centrifugal-based differential migration. Furthermore, sorting cellular organelles by their intrinsic inertia enjoys the benefits of easy operation, undemanding equipment needs and continuous batch processing. Herein, we have successfully isolated functional mitochondria from crude cell lysate of less than 100 cells, which demonstrates the possibilities of promoting this methodology for detailed analyses of subcellular organelles, particularly when small-scale clinically relevant samples are considered.

Original language	English
Journal	R S C Advances
Volume	7
Issue	38
Pages (from-to)	23735-23741
Number of pages	7
ISSN	2046-2069
DOIs	https://doi.org/10.1039/c7ra03384b
Publication status	Published - 3 May 2017

Keywords

ON-A-CHIP
CROSS-PARTITION
CYTOCHROME-C
LIVER
SEPARATION
APOPTOSIS
DEVICE

Access to Document

10.1039/c7ra03384bLicence: CC BY-NC

c7ra03384bFinal published version, 710 KBLicence: CC BY-NC

Cite this

@article{84b644fb96354e2798ef6cda8762921e,

title = "Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample",

abstract = "Isolation of subcellular fractions from a limited amount of clinical sample is imperative for the continuous advancement of biological and clinical research. For example, analyses of pure and functional mitochondria from patient samples are expected not only to expand our knowledge towards the basic biological mechanisms of mitochondrial function, but also to elucidate how mitochondria are involved in the development of diseases such as cancers, premature aging syndromes, diabetes and neurodegenerative disorders. While currently available methods are mostly laborious and not suitable for small-scale analyses, we present a novel and facile approach to isolate mitochondria from limited amounts of biological samples by centrifugal-based differential migration. Furthermore, sorting cellular organelles by their intrinsic inertia enjoys the benefits of easy operation, undemanding equipment needs and continuous batch processing. Herein, we have successfully isolated functional mitochondria from crude cell lysate of less than 100 cells, which demonstrates the possibilities of promoting this methodology for detailed analyses of subcellular organelles, particularly when small-scale clinically relevant samples are considered.",

keywords = "ON-A-CHIP, CROSS-PARTITION, CYTOCHROME-C, LIVER, SEPARATION, APOPTOSIS, DEVICE",

author = "C. Tesauro and B. Ferrando and X. Ma and Jepsen, {M. L.} and Ivarsen, {Anne Kathrine} and R. Frohlich and T. Stevnsner and Knudsen, {B. R.} and Ho, {Y. P.}",

year = "2017",

month = may,

day = "3",

doi = "10.1039/c7ra03384b",

language = "English",

volume = "7",

pages = "23735--23741",

journal = "R S C Advances",

issn = "2046-2069",

publisher = "Royal Society of Chemistry",

number = "38",

}

Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample. / Tesauro, C.; Ferrando, B.; Ma, X. et al.
In: R S C Advances, Vol. 7, No. 38, 03.05.2017, p. 23735-23741.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample

AU - Tesauro, C.

AU - Ferrando, B.

AU - Ma, X.

AU - Jepsen, M. L.

AU - Ivarsen, Anne Kathrine

AU - Frohlich, R.

AU - Stevnsner, T.

AU - Knudsen, B. R.

AU - Ho, Y. P.

PY - 2017/5/3

Y1 - 2017/5/3

N2 - Isolation of subcellular fractions from a limited amount of clinical sample is imperative for the continuous advancement of biological and clinical research. For example, analyses of pure and functional mitochondria from patient samples are expected not only to expand our knowledge towards the basic biological mechanisms of mitochondrial function, but also to elucidate how mitochondria are involved in the development of diseases such as cancers, premature aging syndromes, diabetes and neurodegenerative disorders. While currently available methods are mostly laborious and not suitable for small-scale analyses, we present a novel and facile approach to isolate mitochondria from limited amounts of biological samples by centrifugal-based differential migration. Furthermore, sorting cellular organelles by their intrinsic inertia enjoys the benefits of easy operation, undemanding equipment needs and continuous batch processing. Herein, we have successfully isolated functional mitochondria from crude cell lysate of less than 100 cells, which demonstrates the possibilities of promoting this methodology for detailed analyses of subcellular organelles, particularly when small-scale clinically relevant samples are considered.

AB - Isolation of subcellular fractions from a limited amount of clinical sample is imperative for the continuous advancement of biological and clinical research. For example, analyses of pure and functional mitochondria from patient samples are expected not only to expand our knowledge towards the basic biological mechanisms of mitochondrial function, but also to elucidate how mitochondria are involved in the development of diseases such as cancers, premature aging syndromes, diabetes and neurodegenerative disorders. While currently available methods are mostly laborious and not suitable for small-scale analyses, we present a novel and facile approach to isolate mitochondria from limited amounts of biological samples by centrifugal-based differential migration. Furthermore, sorting cellular organelles by their intrinsic inertia enjoys the benefits of easy operation, undemanding equipment needs and continuous batch processing. Herein, we have successfully isolated functional mitochondria from crude cell lysate of less than 100 cells, which demonstrates the possibilities of promoting this methodology for detailed analyses of subcellular organelles, particularly when small-scale clinically relevant samples are considered.

KW - ON-A-CHIP

KW - CROSS-PARTITION

KW - CYTOCHROME-C

KW - LIVER

KW - SEPARATION

KW - APOPTOSIS

KW - DEVICE

U2 - 10.1039/c7ra03384b

DO - 10.1039/c7ra03384b

M3 - Journal article

SN - 2046-2069

VL - 7

SP - 23735

EP - 23741

JO - R S C Advances

JF - R S C Advances

IS - 38

ER -

Isolation of functional mitochondria by inertial microfluidics - a new method to sort intracellular organelles from a small scale biological sample

Abstract

Keywords

Access to Document

Fingerprint

Cite this