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Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation

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  • Marc Bailly, Denmark
  • Mickael Blaise, Denmark
  • Bernard Lorber, Denmark
  • Søren Skou Thirup
  • Daniel Kern, Denmark
  • Department of Molecular Biology
Thermus thermophilus deprived of asparagine synthetase synthesizes Asn on tRNA(Asn) via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNA(Asn) prior to conversion of the Asp to Asn by GatCAB, a tRNA-dependent amidotransferase. This pathway also constitutes the route of Asn-tRNA(Asn) formation by bacteria and archaea deprived of asparaginyl-tRNA synthetase. The partners involved in tRNA-dependent Asn formation in T. thermophilus assemble into a ternary complex called the transamidosome. This particule produces Asn-tRNA(Asn) in the presence of free Asp, ATP and an amido-group donor. Crystals of the transamidosome from T. thermophilus were obtained in the presence of PEG 4000 in MES-NaOH buffer pH 6.5. They belonged to the primitive monoclinic space group P2(1), with unit-cell parameters a = 115.9, b = 214.0, c = 127.8 A, beta = 93.3 degrees . A complete data set was collected to 3 A resolution. Here, the isolation and crystallization of the transamidosome from T. thermophilus and preliminary crystallographic data are reported.
Original languageEnglish
JournalActa Crystallographica. Section F: Structural Biology and Crystallization Communications Online
Volume65
IssuePt 6
Pages (from-to)577-81
Number of pages4
ISSN2053-230X
DOIs
Publication statusPublished - 2009

    Research areas

  • Asparagine, Aspartate-tRNA Ligase, Crystallization, Data Collection, Escherichia coli, Light, RNA, Transfer, Amino Acyl, RNA, Transfer, Asn, Ribonucleoproteins, Scattering, Radiation, Statistics as Topic, Thermus thermophilus, Transfer RNA Aminoacylation, X-Ray Diffraction

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