Isolation and characterization of an R prime plasmid in Rhizobium meliloti

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Isolation and characterization of an R prime plasmid in Rhizobium meliloti. / Kiss, G B; Dobó, K; Dusha, I; Breznovits, A; Orosz, L; Vincze, Éva; Kondorosi, A.

In: Journal of Bacteriology, Vol. 141, 1980, p. 121-128.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Kiss, GB, Dobó, K, Dusha, I, Breznovits, A, Orosz, L, Vincze, É & Kondorosi, A 1980, 'Isolation and characterization of an R prime plasmid in Rhizobium meliloti', Journal of Bacteriology, vol. 141, pp. 121-128.

APA

Kiss, G. B., Dobó, K., Dusha, I., Breznovits, A., Orosz, L., Vincze, É., & Kondorosi, A. (1980). Isolation and characterization of an R prime plasmid in Rhizobium meliloti. Journal of Bacteriology, 141, 121-128.

CBE

Kiss GB, Dobó K, Dusha I, Breznovits A, Orosz L, Vincze É, Kondorosi A. 1980. Isolation and characterization of an R prime plasmid in Rhizobium meliloti. Journal of Bacteriology. 141:121-128.

MLA

Kiss, G B et al. "Isolation and characterization of an R prime plasmid in Rhizobium meliloti". Journal of Bacteriology. 1980, 141. 121-128.

Vancouver

Kiss GB, Dobó K, Dusha I, Breznovits A, Orosz L, Vincze É et al. Isolation and characterization of an R prime plasmid in Rhizobium meliloti. Journal of Bacteriology. 1980;141:121-128.

Author

Kiss, G B ; Dobó, K ; Dusha, I ; Breznovits, A ; Orosz, L ; Vincze, Éva ; Kondorosi, A. / Isolation and characterization of an R prime plasmid in Rhizobium meliloti. In: Journal of Bacteriology. 1980 ; Vol. 141. pp. 121-128.

Bibtex

@article{365caa50abf611dda608000ea68e967b,
title = "Isolation and characterization of an R prime plasmid in Rhizobium meliloti",
abstract = "Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria",
author = "Kiss, {G B} and K Dob{\'o} and I Dusha and A Breznovits and L Orosz and {\'E}va Vincze and A Kondorosi",
year = "1980",
language = "English",
volume = "141",
pages = "121--128",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",

}

RIS

TY - JOUR

T1 - Isolation and characterization of an R prime plasmid in Rhizobium meliloti

AU - Kiss, G B

AU - Dobó, K

AU - Dusha, I

AU - Breznovits, A

AU - Orosz, L

AU - Vincze, Éva

AU - Kondorosi, A

PY - 1980

Y1 - 1980

N2 - Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria

AB - Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria

M3 - Journal article

VL - 141

SP - 121

EP - 128

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

ER -