Isolation and characterization of an R prime plasmid in Rhizobium meliloti

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  • G B Kiss, Denmark
  • K Dobó, Denmark
  • I Dusha, Denmark
  • A Breznovits, Denmark
  • L Orosz, Denmark
  • Éva Vincze
  • A Kondorosi, Denmark
Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria
Original languageEnglish
JournalJournal of Bacteriology
Pages (from-to)121-128
Number of pages8
Publication statusPublished - 1980
Externally publishedYes

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