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Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation

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  • Mamadou Soumboundou, Laboratoire Biophysique UFR-Santé
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  • Julien Dossou, Département du Génie d'Imagerie Médicale et Radiobiologie
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  • Yossef Kalaga, Centre Hospitalier Yalgado Radioprotection-Radiobiologie
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  • Innocent Nkengurutse, Laboratoire de Radiobiologie et d'Oncologie, IRCM/DSV/CEA, 92265 Fontenay aux Roses, France. cuceu_corina@yahoo.com.
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  • Ibrahima Faye, Cell Environment
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  • Albert Guingani, Centre Hospitalier Yalgado Radioprotection-Radiobiologie
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  • Macoura Gadji, Service Hématologie UCAD
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  • Koudbi J Yameogo, Centre Hospitalier Yalgado Radioprotection-Radiobiologie
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  • Henri Zongo, Centre Hospitalier Yalgado Radioprotection-Radiobiologie
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  • Gora Mbaye, Laboratoire Biophysique UFR-Santé
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  • Ahmadou Dem, Service Hématologie UCAD
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  • Mounibé Diarra, Laboratoire Biophysique UFR-Santé
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  • Rached Adjibade, Département du Génie d'Imagerie Médicale et Radiobiologie
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  • Catherine Djebou, Département du Génie d'Imagerie Médicale et Radiobiologie
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  • Steffen Junker
  • Noufissa Oudrhiri, Inserm, CESP, U1018, Villejuif, France; Univ Paris-Sud, UMRS 1018, Villejuif, France; Gustave Roussy Institute, F-94800 Villejuif, France.
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  • William M Hempel, Cell Environment
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  • Alain Dieterlen, IRIMAS, Institut de Recherche en Informatique, Mathématiques, Automatique et Signal, Université de Haute-Alsace, 68093 Mulhouse, France. bruno.colicchio@uha.fr.
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  • Eric Jeandidier, Service de Génétique Groupe Hospitalier de la Région de Mulhouse et Sud Alsace Mulhouse
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  • Patrice Carde, Capitol Region of Denmark
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  • Elie El Maalouf, Cell Environment
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  • Bruno Colicchio, IRIMAS, Institut de Recherche en Informatique, Mathématiques, Automatique et Signal, Université de Haute-Alsace, 68093 Mulhouse, France. bruno.colicchio@uha.fr.
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  • Annelise Bennaceur-Griscelli, Inserm, CESP, U1018, Villejuif, France; Univ Paris-Sud, UMRS 1018, Villejuif, France; Gustave Roussy Institute, F-94800 Villejuif, France.
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  • Michael Fenech, Univ South Australia, University of South Australia, Future Ind Inst
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  • Philippe Voisin, Cell Environment
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  • Claire Rodriguez-Lafrasse, Laboratoire de Radiobiologie Cellulaire et Moléculaire
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  • Radhia M'Kacher, Cell Environment

Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry. Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0-4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations. Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10-17), centric rings (p = 4.0310-14), and resulting double-strand-breaks (DSB) (p = 1.32 10-18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa. Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.

Original languageEnglish
Article number657999
JournalFrontiers in Genetics
Volume12
ISSN1664-8021
DOIs
Publication statusPublished - 11 Nov 2021

Bibliographical note

Copyright © 2021 Soumboundou, Dossou, Kalaga, Nkengurutse, Faye, Guingani, Gadji, Yameogo, Zongo, Mbaye, Dem, Diarra, Adjibade, Djebou, Junker, Oudrhiri, Hempel, Dieterlen, Jeandidier, Carde, El Maalouf, Colicchio, Bennaceur-Griscelli, Fenech, Voisin, Rodriguez-Lafrasse and M’Kacher.

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