Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry?: A Study of Dose-Response After in vitro Irradiation

Mamadou Soumboundou; Julien Dossou; Yossef Kalaga; Innocent Nkengurutse; Ibrahima Faye; Albert Guingani; Macoura Gadji; Koudbi J Yameogo; Henri Zongo; Gora Mbaye; Ahmadou Dem; Mounibé Diarra; Rached Adjibade; Catherine Djebou; Steffen Junker; Noufissa Oudrhiri; William M Hempel; Alain Dieterlen; Eric Jeandidier; Patrice Carde; Elie El Maalouf; Bruno Colicchio; Annelise Bennaceur-Griscelli; Michael Fenech; Philippe Voisin; Claire Rodriguez-Lafrasse; Radhia M'Kacher

doi:10.3389/fgene.2021.657999

Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation

Mamadou Soumboundou, Julien Dossou, Yossef Kalaga, Innocent Nkengurutse, Ibrahima Faye, Albert Guingani, Macoura Gadji, Koudbi J Yameogo, Henri Zongo, Gora Mbaye, Ahmadou Dem, Mounibé Diarra, Rached Adjibade, Catherine Djebou, Steffen Junker, Noufissa Oudrhiri, William M Hempel, Alain Dieterlen, Eric Jeandidier, Patrice CardeElie El Maalouf, Bruno Colicchio, Annelise Bennaceur-Griscelli, Michael Fenech, Philippe Voisin, Claire Rodriguez-Lafrasse, Radhia M'Kacher

Department of Biomedicine - Forskning og uddannelse, Vest

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

1 Citation (Scopus)

Abstract

Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry. Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0-4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations. Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10-17), centric rings (p = 4.0310-14), and resulting double-strand-breaks (DSB) (p = 1.32 10-18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa. Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.

Original language	English
Article number	657999
Journal	Frontiers in Genetics
Volume	12
ISSN	1664-8021
DOIs	https://doi.org/10.3389/fgene.2021.657999
Publication status	Published - 11 Nov 2021

Keywords

African donors
European donors
centromeres
dicentric chromosome
irradiation
telomeres

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10.3389/fgene.2021.657999

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Soumboundou, M., Dossou, J., Kalaga, Y., Nkengurutse, I., Faye, I., Guingani, A., Gadji, M., Yameogo, K. J., Zongo, H., Mbaye, G., Dem, A., Diarra, M., Adjibade, R., Djebou, C., Junker, S., Oudrhiri, N., Hempel, W. M., Dieterlen, A., Jeandidier, E., ... M'Kacher, R. (2021). Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation. Frontiers in Genetics, 12, Article 657999. https://doi.org/10.3389/fgene.2021.657999

@article{35a7f760e7a347fbacf65b187d8620f2,

title = "Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry?: A Study of Dose-Response After in vitro Irradiation",

abstract = "Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry. Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0-4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations. Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10-17), centric rings (p = 4.0310-14), and resulting double-strand-breaks (DSB) (p = 1.32 10-18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa. Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.",

keywords = "African donors, European donors, centromeres, dicentric chromosome, irradiation, telomeres",

author = "Mamadou Soumboundou and Julien Dossou and Yossef Kalaga and Innocent Nkengurutse and Ibrahima Faye and Albert Guingani and Macoura Gadji and Yameogo, {Koudbi J} and Henri Zongo and Gora Mbaye and Ahmadou Dem and Mounib{\'e} Diarra and Rached Adjibade and Catherine Djebou and Steffen Junker and Noufissa Oudrhiri and Hempel, {William M} and Alain Dieterlen and Eric Jeandidier and Patrice Carde and {El Maalouf}, Elie and Bruno Colicchio and Annelise Bennaceur-Griscelli and Michael Fenech and Philippe Voisin and Claire Rodriguez-Lafrasse and Radhia M'Kacher",

note = "Copyright {\textcopyright} 2021 Soumboundou, Dossou, Kalaga, Nkengurutse, Faye, Guingani, Gadji, Yameogo, Zongo, Mbaye, Dem, Diarra, Adjibade, Djebou, Junker, Oudrhiri, Hempel, Dieterlen, Jeandidier, Carde, El Maalouf, Colicchio, Bennaceur-Griscelli, Fenech, Voisin, Rodriguez-Lafrasse and M{\textquoteright}Kacher.",

year = "2021",

month = nov,

day = "11",

doi = "10.3389/fgene.2021.657999",

language = "English",

volume = "12",

journal = "Frontiers in Genetics",

issn = "1664-8021",

publisher = "Frontiers Media SA",

}

Soumboundou, M, Dossou, J, Kalaga, Y, Nkengurutse, I, Faye, I, Guingani, A, Gadji, M, Yameogo, KJ, Zongo, H, Mbaye, G, Dem, A, Diarra, M, Adjibade, R, Djebou, C, Junker, S, Oudrhiri, N, Hempel, WM, Dieterlen, A, Jeandidier, E, Carde, P, El Maalouf, E, Colicchio, B, Bennaceur-Griscelli, A, Fenech, M, Voisin, P, Rodriguez-Lafrasse, C & M'Kacher, R 2021, 'Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation', Frontiers in Genetics, vol. 12, 657999. https://doi.org/10.3389/fgene.2021.657999

Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation. / Soumboundou, Mamadou; Dossou, Julien; Kalaga, Yossef et al.
In: Frontiers in Genetics, Vol. 12, 657999, 11.11.2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry?

T2 - A Study of Dose-Response After in vitro Irradiation

AU - Soumboundou, Mamadou

AU - Dossou, Julien

AU - Kalaga, Yossef

AU - Nkengurutse, Innocent

AU - Faye, Ibrahima

AU - Guingani, Albert

AU - Gadji, Macoura

AU - Yameogo, Koudbi J

AU - Zongo, Henri

AU - Mbaye, Gora

AU - Dem, Ahmadou

AU - Diarra, Mounibé

AU - Adjibade, Rached

AU - Djebou, Catherine

AU - Junker, Steffen

AU - Oudrhiri, Noufissa

AU - Hempel, William M

AU - Dieterlen, Alain

AU - Jeandidier, Eric

AU - Carde, Patrice

AU - El Maalouf, Elie

AU - Colicchio, Bruno

AU - Bennaceur-Griscelli, Annelise

AU - Fenech, Michael

AU - Voisin, Philippe

AU - Rodriguez-Lafrasse, Claire

AU - M'Kacher, Radhia

N1 - Copyright © 2021 Soumboundou, Dossou, Kalaga, Nkengurutse, Faye, Guingani, Gadji, Yameogo, Zongo, Mbaye, Dem, Diarra, Adjibade, Djebou, Junker, Oudrhiri, Hempel, Dieterlen, Jeandidier, Carde, El Maalouf, Colicchio, Bennaceur-Griscelli, Fenech, Voisin, Rodriguez-Lafrasse and M’Kacher.

PY - 2021/11/11

Y1 - 2021/11/11

N2 - Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry. Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0-4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations. Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10-17), centric rings (p = 4.0310-14), and resulting double-strand-breaks (DSB) (p = 1.32 10-18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa. Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.

AB - Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry. Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0-4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations. Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10-17), centric rings (p = 4.0310-14), and resulting double-strand-breaks (DSB) (p = 1.32 10-18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa. Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.

KW - African donors

KW - European donors

KW - centromeres

KW - dicentric chromosome

KW - irradiation

KW - telomeres

UR - http://www.scopus.com/inward/record.url?scp=85120704169&partnerID=8YFLogxK

U2 - 10.3389/fgene.2021.657999

DO - 10.3389/fgene.2021.657999

M3 - Journal article

C2 - 34868192

SN - 1664-8021

VL - 12

JO - Frontiers in Genetics

JF - Frontiers in Genetics

M1 - 657999

ER -

Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation

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