TY - JOUR
T1 - Inulin Supplementation Modulates the Hepatic Transcriptome, Metabolome, and Ferritin Content in Ovariectomized Rats
AU - Zhao, Xiaorui
AU - He, Weiwei
AU - Jakobsen, Louise Margrethe Arildsen
AU - Zachariassen, Line Fisker
AU - Hansen, Axel K.
AU - Rasmussen, Martin Krøyer
AU - Bertram, Hanne Christine S.
PY - 2023/12
Y1 - 2023/12
N2 - Scope: Liver is an important metabolic organ regulating whole-body homeostasis. This study aims to investigate how prebiotic-induced changes in the metabolic activity of the gut microbiome (GM) and dietary calcium depletion modulates the hepatic metabolome and transcriptome. Methods and results: The serum metabolome, liver metabolome, and transcriptome are determined on samples from ovariectomized (OVX) rats fed a control diet (Control, n = 7), a control diet supplemented with 5% w/w inulin (Inulin, n = 7), or a calcium-deficient diet (CaDef, n = 7). Inulin fortification is associated with higher serum concentrations of acetate, 3-hydroxybutyrate, and reduced concentration of dimethyl sulfone, revealing that changes in the metabolic activity of the GM are reflected in circulating metabolites. Metabolomics also reveal that the inulin-fortified diet results in lower concentrations of hepatic glutamate, serine, and hypoxanthine while transcriptomics reveal accompanying effects on the hepatic expression of ferric iron binding-related genes. Inulin fortification also induces effects on the hepatic expression of genes involved in olfactory transduction, suggesting that prebiotics regulate liver function through yet unidentified mechanisms involving olfactory receptors. Conclusion: Inulin ingestion impacts hepatic gene expression and is associated with an upregulation of ferritin synthesis-related genes and liver ferritin content.
AB - Scope: Liver is an important metabolic organ regulating whole-body homeostasis. This study aims to investigate how prebiotic-induced changes in the metabolic activity of the gut microbiome (GM) and dietary calcium depletion modulates the hepatic metabolome and transcriptome. Methods and results: The serum metabolome, liver metabolome, and transcriptome are determined on samples from ovariectomized (OVX) rats fed a control diet (Control, n = 7), a control diet supplemented with 5% w/w inulin (Inulin, n = 7), or a calcium-deficient diet (CaDef, n = 7). Inulin fortification is associated with higher serum concentrations of acetate, 3-hydroxybutyrate, and reduced concentration of dimethyl sulfone, revealing that changes in the metabolic activity of the GM are reflected in circulating metabolites. Metabolomics also reveal that the inulin-fortified diet results in lower concentrations of hepatic glutamate, serine, and hypoxanthine while transcriptomics reveal accompanying effects on the hepatic expression of ferric iron binding-related genes. Inulin fortification also induces effects on the hepatic expression of genes involved in olfactory transduction, suggesting that prebiotics regulate liver function through yet unidentified mechanisms involving olfactory receptors. Conclusion: Inulin ingestion impacts hepatic gene expression and is associated with an upregulation of ferritin synthesis-related genes and liver ferritin content.
KW - NMR-based metabolomics
KW - dietary fiber
KW - gut-liver axis
KW - liver profiling
KW - mineral absorption
KW - prebiotics
KW - Transcriptome
KW - Metabolome
KW - Rats
KW - Prebiotics
KW - Animals
KW - Liver/metabolism
KW - Inulin/pharmacology
KW - Dietary Supplements
UR - http://www.scopus.com/inward/record.url?scp=85174291709&partnerID=8YFLogxK
U2 - 10.1002/mnfr.202300372
DO - 10.1002/mnfr.202300372
M3 - Journal article
C2 - 37849247
SN - 1613-4125
VL - 67
JO - Molecular Nutrition & Food Research
JF - Molecular Nutrition & Food Research
IS - 23
M1 - 2300372
ER -