Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants

Pankaj Sehgal; Hidekazu Doe; Manu Sharma; Daniel E. Otzen

doi:10.1007/s00396-003-0992-0

Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants

Pankaj Sehgal^*, Hidekazu Doe, Manu Sharma, Daniel E. Otzen

^*Corresponding author for this work

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

5 Citations (Scopus)

Abstract

Solubilization of different zwitterionic phospholipid vesicles structures such as L-α-phosphatidyl-choline (PC) and 1,2-didecanoyl-snglycero-3-phosphocholine (DPC) have been studied in aqueous bulk by using zwitterionic surfactant dimethylhexadecylammoniopropanesulfonate (HPS). This has been done by studying the aggregation of HPS in pure water and in the presence of 7-36 μM of fixed concentrations of each lipid with the help of pyrene fluorescence intensity (I₁/I₃) measurements. The fluorescence measurements showed that HPS monomers undergo two kinds of aggregation process, which were identified by the three breaks in a plot of pyrene fluorescence versus HPS concentration. The first two breaks, C₁ and C₂, indicate the onset and completion of vesicle solubilization respectively, upon incorporation of HPS monomers into the vesicles and led to solubilization in the form of mixed micelles. This process was not clearly visible at low lipid concentration. We evaluated the partition coefficient (K), which defines the degree of partitioning of surfactant monomers into the vesicles with respect to the aqueous medium. A high K value of HPS-lipid aggregates indicates the stronger interactions between surfactant and lipid vesicles. The K values evaluated for PC and DPC are quite close to each other, which indicates that K values were independent of phospholipid chain length.

Original language	English
Book series	Colloid and Polymer Science
Volume	282
Issue	7
Pages (from-to)	677-683
Number of pages	7
ISSN	0303-402X
DOIs	https://doi.org/10.1007/s00396-003-0992-0
Publication status	Published - 1 May 2004

Keywords

Bulk properties
Critical micellar concentration
Mixed micelles
Surfactant-lipid interaction
Vesicles

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10.1007/s00396-003-0992-0

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title = "Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants",

abstract = "Solubilization of different zwitterionic phospholipid vesicles structures such as L-α-phosphatidyl-choline (PC) and 1,2-didecanoyl-snglycero-3-phosphocholine (DPC) have been studied in aqueous bulk by using zwitterionic surfactant dimethylhexadecylammoniopropanesulfonate (HPS). This has been done by studying the aggregation of HPS in pure water and in the presence of 7-36 μM of fixed concentrations of each lipid with the help of pyrene fluorescence intensity (I1/I3) measurements. The fluorescence measurements showed that HPS monomers undergo two kinds of aggregation process, which were identified by the three breaks in a plot of pyrene fluorescence versus HPS concentration. The first two breaks, C1 and C2, indicate the onset and completion of vesicle solubilization respectively, upon incorporation of HPS monomers into the vesicles and led to solubilization in the form of mixed micelles. This process was not clearly visible at low lipid concentration. We evaluated the partition coefficient (K), which defines the degree of partitioning of surfactant monomers into the vesicles with respect to the aqueous medium. A high K value of HPS-lipid aggregates indicates the stronger interactions between surfactant and lipid vesicles. The K values evaluated for PC and DPC are quite close to each other, which indicates that K values were independent of phospholipid chain length.",

keywords = "Bulk properties, Critical micellar concentration, Mixed micelles, Surfactant-lipid interaction, Vesicles",

author = "Pankaj Sehgal and Hidekazu Doe and Manu Sharma and Otzen, {Daniel E.}",

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Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants. / Sehgal, Pankaj; Doe, Hidekazu; Sharma, Manu et al.
In: Colloid and Polymer Science, Vol. 282, No. 7, 01.05.2004, p. 677-683.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants

AU - Sehgal, Pankaj

AU - Doe, Hidekazu

AU - Sharma, Manu

AU - Otzen, Daniel E.

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Solubilization of different zwitterionic phospholipid vesicles structures such as L-α-phosphatidyl-choline (PC) and 1,2-didecanoyl-snglycero-3-phosphocholine (DPC) have been studied in aqueous bulk by using zwitterionic surfactant dimethylhexadecylammoniopropanesulfonate (HPS). This has been done by studying the aggregation of HPS in pure water and in the presence of 7-36 μM of fixed concentrations of each lipid with the help of pyrene fluorescence intensity (I1/I3) measurements. The fluorescence measurements showed that HPS monomers undergo two kinds of aggregation process, which were identified by the three breaks in a plot of pyrene fluorescence versus HPS concentration. The first two breaks, C1 and C2, indicate the onset and completion of vesicle solubilization respectively, upon incorporation of HPS monomers into the vesicles and led to solubilization in the form of mixed micelles. This process was not clearly visible at low lipid concentration. We evaluated the partition coefficient (K), which defines the degree of partitioning of surfactant monomers into the vesicles with respect to the aqueous medium. A high K value of HPS-lipid aggregates indicates the stronger interactions between surfactant and lipid vesicles. The K values evaluated for PC and DPC are quite close to each other, which indicates that K values were independent of phospholipid chain length.

AB - Solubilization of different zwitterionic phospholipid vesicles structures such as L-α-phosphatidyl-choline (PC) and 1,2-didecanoyl-snglycero-3-phosphocholine (DPC) have been studied in aqueous bulk by using zwitterionic surfactant dimethylhexadecylammoniopropanesulfonate (HPS). This has been done by studying the aggregation of HPS in pure water and in the presence of 7-36 μM of fixed concentrations of each lipid with the help of pyrene fluorescence intensity (I1/I3) measurements. The fluorescence measurements showed that HPS monomers undergo two kinds of aggregation process, which were identified by the three breaks in a plot of pyrene fluorescence versus HPS concentration. The first two breaks, C1 and C2, indicate the onset and completion of vesicle solubilization respectively, upon incorporation of HPS monomers into the vesicles and led to solubilization in the form of mixed micelles. This process was not clearly visible at low lipid concentration. We evaluated the partition coefficient (K), which defines the degree of partitioning of surfactant monomers into the vesicles with respect to the aqueous medium. A high K value of HPS-lipid aggregates indicates the stronger interactions between surfactant and lipid vesicles. The K values evaluated for PC and DPC are quite close to each other, which indicates that K values were independent of phospholipid chain length.

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KW - Critical micellar concentration

KW - Mixed micelles

KW - Surfactant-lipid interaction

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ER -

Interaction between phospholipid vesicular structures with long chain zwitterionic surfactants

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