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Integrative whole-brain neuroscience in larval zebrafish

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  • Gilles C. Vanwalleghem
  • Misha B. Ahrens, Howard Hughes Medical Institute
  • ,
  • Ethan K. Scott, University of Queensland

Due to their small size and transparency, zebrafish larvae are amenable to a range of fluorescence microscopy techniques. With the development of sensitive genetically encoded calcium indicators, this has extended to the whole-brain imaging of neural activity with cellular resolution. This technique has been used to study brain-wide population dynamics accompanying sensory processing and sensorimotor transformations, and has spurred the development of innovative closed-loop behavioral paradigms in which stimulus–response relationships can be studied. More recently, microscopes have been developed that allow whole-brain calcium imaging in freely swimming and behaving larvae. In this review, we highlight the technologies underlying whole-brain functional imaging in zebrafish, provide examples of the sensory and motor processes that have been studied with this technique, and discuss the need to merge data from whole-brain functional imaging studies with neurochemical and anatomical information to develop holistic models of functional neural circuits.

Original languageEnglish
JournalCurrent Opinion in Neurobiology
Pages (from-to)136-145
Number of pages10
Publication statusPublished - Jun 2018
Externally publishedYes

Bibliographical note

Funding Information:
Support was provided by the NHMRC Project Grant ( APP1066887 ), ARC Future Fellowship ( FT110100887 ), a grant from the Simons Foundation ( SFARI 399432 ), and two ARC Discovery Project Grants ( DP140102036 & DP110103612 ) to EKS; and an EMBO Long-term Fellowship to GCV. MBA is supported by the Howard Hughes Medical Institute.

Publisher Copyright:
© 2018 Elsevier Ltd

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