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Integrated Analysis of mRNA and MicroRNA Co-expressed Network for the Differentiation of Bovine Skeletal Muscle Cells After Polyphenol Resveratrol Treatment

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  • Dan Hao, Northwest Agriculture and Forestry University
  • ,
  • Xiao Wang, Konge Larsen ApS
  • ,
  • Yu Yang, Northwest Agriculture and Forestry University
  • ,
  • Bo Thomsen
  • Lars Erik Holm
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  • Kaixing Qu, Chuxiong Normal University
  • ,
  • Bizhi Huang, Yunnan Academy of Grassland and Animal Science
  • ,
  • Hong Chen, Northwest Agriculture and Forestry University, Xinjiang Agriculture University

Resveratrol (RSV) has been confirmed to benefit human health. Resveratrol supplemented in the feeds of animals improved pork, chicken, and duck meat qualities. In this study, we identified differentially expressed (DE) messenger RNAs (mRNAs) (n = 3,856) and microRNAs (miRNAs) (n = 93) for the weighted gene co-expression network analysis (WGCNA) to investigate the co-expressed DE mRNAs and DE miRNAs in the primary bovine myoblasts after RSV treatment. The mRNA results indicated that RSV treatments had high correlations with turquoise module (0.91, P-value = 0.01) and blue module (0.93, P-value < 0.01), while only the turquoise module (0.96, P-value < 0.01) was highly correlated with the treatment status using miRNA data. After biological enrichment analysis, the 2,579 DE genes in the turquoise module were significantly enriched in the Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The top two GO terms were actin filament-based process (GO:0030029) and actin cytoskeleton organization (GO:0030036). The top two KEGG pathways were regulation of actin cytoskeleton (bta04810) and tight junction (bta04530). Then, we constructed the DE mRNA co-expression and DE miRNA co-expression networks in the turquoise module and the mRNA–miRNA targeting networks based on their co-expressions in the key module. In summary, the RSV-induced miRNAs participated in the co-expression networks that could affect mRNA expressions to regulate the primary myoblast differentiation. Our study provided a better understanding of the roles of RSV in inducing miRNA and of the characteristics of DE miRNAs in the key co-expressed module in regulation of mRNAs and revealed new candidate regulatory miRNAs and genes for the beef quality traits.

Original languageEnglish
Article number777477
JournalFrontiers in Veterinary Science
Publication statusPublished - Dec 2021

Bibliographical note

Publisher Copyright:
Copyright © 2021 Hao, Wang, Yang, Thomsen, Holm, Qu, Huang and Chen.

    Research areas

  • differentially expressed analysis, miRNA co-expression, mRNA co-expression, mRNA-miRNA network, primary bovine myoblast, resveratrol, WGCNA

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