Inhibitors of α-Synuclein fibrillation and oligomer toxicity in Rosa damascena: the all-pervading powers of flavonoids and phenolic glycosides

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  • Hoda Eskandari
  • ,
  • Mustafa Ghanadian
  • ,
  • Clarice Noleto-Dias
  • ,
  • Charlotte Lomax
  • ,
  • Ahmed Tawfike
  • ,
  • Gunna Christiansen
  • ,
  • Duncan S Sutherland
  • Jane L Ward
  • ,
  • Hossein Mohammad-Beigi
  • ,
  • Daniel Erik Otzen

There is an intense search for natural compounds that can inhibit the oligomerization and fibrillation of α-Synuclein (α-Syn), whose aggregation is key to the development of Parkinson's disease (PD). Rosa damascena is a medicinal herb widely used in Middle Eastern food, ceremonies, and perfumes. The herb is known to contain many different polyphenols. Here we investigated the existence of α-Syn fibrillation inhibitors in R. damascena extract. Different HPLC fractions of the extract were assessed in α-Syn fibrillation and toxicity assays. The most active fractions led to the formation of more α-Syn oligomers but with less toxicity to SH-SY5Y cells, according to MTT and LDH assays. LC-MS analysis identified gallic acid, kaempferol 3-glucoside, kaempferol-3-O-β-rutinoside, and quercetin which were subsequently shown to be strong α-Syn fibrillation inhibitors. Our results highlight the benefits of R. damascena extract to combat PD at the population level.

Original languageEnglish
JournalACS Chemical Neuroscience
Volume11
Issue19
Pages (from-to)3161–3173
ISSN1948-7193
DOIs
Publication statusPublished - Oct 2020

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