Abstract
There is an intense search for natural compounds that can inhibit the oligomerization and fibrillation of α-Synuclein (α-Syn), whose aggregation is key to the development of Parkinson's disease (PD). Rosa damascena is a medicinal herb widely used in Middle Eastern food, ceremonies, and perfumes. The herb is known to contain many different polyphenols. Here we investigated the existence of α-Syn fibrillation inhibitors in R. damascena extract. Different HPLC fractions of the extract were assessed in α-Syn fibrillation and toxicity assays. The most active fractions led to the formation of more α-Syn oligomers but with less toxicity to SH-SY5Y cells, according to MTT and LDH assays. LC-MS analysis identified gallic acid, kaempferol 3-glucoside, kaempferol-3-O-β-rutinoside, and quercetin which were subsequently shown to be strong α-Syn fibrillation inhibitors. Our results highlight the benefits of R. damascena extract to combat PD at the population level.
Original language | English |
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Journal | ACS Chemical Neuroscience |
Volume | 11 |
Issue | 19 |
Pages (from-to) | 3161–3173 |
Number of pages | 13 |
ISSN | 1948-7193 |
DOIs | |
Publication status | Published - 7 Oct 2020 |
Keywords
- Amyloid
- Cell toxicity
- Neurodegeneration
- Oligomerization
- Parkinson's disease
- Phenolics
- Plant
- Rosa damascena
- Sugars
- α-Synuclein