Influence of diurnal photosynthetic activity on the morphology, structure, and thermal properties of normal and waxy barley starch

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  • Avi Goldstein, Department of Food Science and Nutrition, University of Minnesota, St Paul, MN 55108, USA.
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  • George Annor, Department of Food Science and Nutrition, University of Minnesota, St Paul, MN 55108, USA.
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  • Varatharajan Vamadevan, Department of Food Science and Nutrition, University of Minnesota, St Paul, MN 55108, USA.
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  • Ian Tetlow, Department of Molecular and Cellular Biology, Summerlee Science Complex, University of Guelph, N1G2W1, Canada.
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  • Jacob J K Kirkensgaard, Niels Bohr Institute, University of Copenhagen,Universitetsparken 5, 2100 Copenhagen, Denmark.
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  • Kell Mortensen, Niels Bohr Institute, University of Copenhagen,Universitetsparken 5, 2100 Copenhagen, Denmark.
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  • Andreas Blennow, Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.
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  • Kim H Hebelstrup
  • Eric Bertoft, Department of Food Science and Nutrition, University of Minnesota, St Paul, MN 55108, USA. Electronic address: eric.bertoft@abo.fi.
This study investigated the influence of diurnal photosynthetic activity on the morphology, molecular composition, crystallinity, and gelatinization properties of normal barley starch (NBS) and waxy barley starch (WBS) granules from plants cultivated in a greenhouse under normal diurnal (16 h light) or constant light photosynthetic conditions. Growth rings were observed in all starch samples regardless of lighting conditions. The size distribution of whole and debranched WBS analyzed by gel-permeation chromatography did not appear to be influenced by the different lighting regimes, however, a greater relative crystallinity measured by wide-angle X-ray scattering and greater crystalline quality as judged by differential scanning calorimetry was observed under the diurnal lighting regime. NBS cultivated under the diurnal photosynthetic lighting regime displayed lower amylose content (18.7%), and shorter amylose chains than its counterpart grown under constant light. Although the relative crystallinity of NBS was not influenced by lighting conditions, lower onset, peak, and completion gelatinization temperatures were observed in diurnally grown NBS compared to constant light conditions. It is concluded that normal barley starch is less influenced by the diurnal photosynthetic lighting regime than amylose-free barley starch suggesting a role of amylose to prevent structural disorder and increase starch granule robustness against environmental cues.
Original languageEnglish
JournalInternational Journal of Biological Macromolecules
Volume98
Pages (from-to)188-200
Number of pages13
ISSN0141-8130
DOIs
Publication statusPublished - May 2017

    Research areas

  • Starch granules, waxy starch, Diurnal activity, Barley

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