Induced Human-like Coronary Stenosis in Hypercholesterolemic PCSK9 Minipigs

Jacob Nicolaisen; Christian Frøsig Bo Poulsen; Martin Mæng Bjørklund; Martin Nors Skov; Maiken Kudahl Larsen; Troels Thim; Jouke Dijkstra; Jacob Fog Bentzon; Evald Høj Christiansen; Niels Ramsing Holm

doi:10.1007/s12265-025-10607-0

Induced Human-like Coronary Stenosis in Hypercholesterolemic PCSK9 Minipigs

Jacob Nicolaisen^*, Christian Frøsig Bo Poulsen, Martin Mæng Bjørklund, Martin Nors Skov, Maiken Kudahl Larsen, Troels Thim, Jouke Dijkstra, Jacob Fog Bentzon, Evald Høj Christiansen, Niels Ramsing Holm

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Translational models for obstructive coronary artery disease are lacking. We aimed to develop a porcine model for obstructive coronary stenosis induced by bioresorbable stent (BRS) implantation in hypercholesterolemic proprotein convertase subtilisin/kexin-9 (PCSK9) minipigs. Fifteen hypercholesterolemic PCSK9 minipigs were randomized to percutaneous coronary intervention with Magmaris, Absorb or Desolve BRS. Optical coherence tomography (OCT) scans were performed at baseline and 6-months followed by histology. Matched OCT analysis showed minimal lumen area decreased from 7.62 ± 1.54 mm² at baseline to 2.12 ± 0.92 mm² at follow-up in the Magmaris group, from 6.99 ± 1.49 mm² to 3.07 ± 1.52 mm² in the Absorb group, and from 6.05 ± 1.11 mm² to 2.65 ± 0.93 mm² in the Desolve group. Histologic examination revealed advanced human-like stenosis. BRS implantation in hypercholesterolemic PCSK9 minipigs induced human-like stenoses and may serve as a feasible preclinical model for obstructive coronary artery disease.

Original language	English
Journal	Journal of Cardiovascular Translational Research
ISSN	1937-5387
DOIs	https://doi.org/10.1007/s12265-025-10607-0
Publication status	Accepted/In press - 2025

Keywords

Animal model
Atherosclerosis
Bioresorbable stent
Fibroatheroma
Obstructive coronary stenosis
Percutaneous coronary intervention

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10.1007/s12265-025-10607-0

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title = "Induced Human-like Coronary Stenosis in Hypercholesterolemic PCSK9 Minipigs",

abstract = "Translational models for obstructive coronary artery disease are lacking. We aimed to develop a porcine model for obstructive coronary stenosis induced by bioresorbable stent (BRS) implantation in hypercholesterolemic proprotein convertase subtilisin/kexin-9 (PCSK9) minipigs. Fifteen hypercholesterolemic PCSK9 minipigs were randomized to percutaneous coronary intervention with Magmaris, Absorb or Desolve BRS. Optical coherence tomography (OCT) scans were performed at baseline and 6-months followed by histology. Matched OCT analysis showed minimal lumen area decreased from 7.62 ± 1.54 mm2 at baseline to 2.12 ± 0.92 mm2 at follow-up in the Magmaris group, from 6.99 ± 1.49 mm2 to 3.07 ± 1.52 mm2 in the Absorb group, and from 6.05 ± 1.11 mm2 to 2.65 ± 0.93 mm2 in the Desolve group. Histologic examination revealed advanced human-like stenosis. BRS implantation in hypercholesterolemic PCSK9 minipigs induced human-like stenoses and may serve as a feasible preclinical model for obstructive coronary artery disease.",

keywords = "Animal model, Atherosclerosis, Bioresorbable stent, Fibroatheroma, Obstructive coronary stenosis, Percutaneous coronary intervention",

author = "Jacob Nicolaisen and Poulsen, {Christian Fr{\o}sig Bo} and Bj{\o}rklund, {Martin M{\ae}ng} and Skov, {Martin Nors} and Larsen, {Maiken Kudahl} and Troels Thim and Jouke Dijkstra and Bentzon, {Jacob Fog} and Christiansen, {Evald H{\o}j} and Holm, {Niels Ramsing}",

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year = "2025",

doi = "10.1007/s12265-025-10607-0",

language = "English",

journal = "Journal of Cardiovascular Translational Research",

issn = "1937-5387",

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Induced Human-like Coronary Stenosis in Hypercholesterolemic PCSK9 Minipigs. / Nicolaisen, Jacob; Poulsen, Christian Frøsig Bo ; Bjørklund, Martin Mæng et al.
In: Journal of Cardiovascular Translational Research, 2025.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Induced Human-like Coronary Stenosis in Hypercholesterolemic PCSK9 Minipigs

AU - Nicolaisen, Jacob

AU - Poulsen, Christian Frøsig Bo

AU - Bjørklund, Martin Mæng

AU - Skov, Martin Nors

AU - Larsen, Maiken Kudahl

AU - Thim, Troels

AU - Dijkstra, Jouke

AU - Bentzon, Jacob Fog

AU - Christiansen, Evald Høj

AU - Holm, Niels Ramsing

N1 - Publisher Copyright: © The Author(s) 2025.

PY - 2025

Y1 - 2025

N2 - Translational models for obstructive coronary artery disease are lacking. We aimed to develop a porcine model for obstructive coronary stenosis induced by bioresorbable stent (BRS) implantation in hypercholesterolemic proprotein convertase subtilisin/kexin-9 (PCSK9) minipigs. Fifteen hypercholesterolemic PCSK9 minipigs were randomized to percutaneous coronary intervention with Magmaris, Absorb or Desolve BRS. Optical coherence tomography (OCT) scans were performed at baseline and 6-months followed by histology. Matched OCT analysis showed minimal lumen area decreased from 7.62 ± 1.54 mm2 at baseline to 2.12 ± 0.92 mm2 at follow-up in the Magmaris group, from 6.99 ± 1.49 mm2 to 3.07 ± 1.52 mm2 in the Absorb group, and from 6.05 ± 1.11 mm2 to 2.65 ± 0.93 mm2 in the Desolve group. Histologic examination revealed advanced human-like stenosis. BRS implantation in hypercholesterolemic PCSK9 minipigs induced human-like stenoses and may serve as a feasible preclinical model for obstructive coronary artery disease.

AB - Translational models for obstructive coronary artery disease are lacking. We aimed to develop a porcine model for obstructive coronary stenosis induced by bioresorbable stent (BRS) implantation in hypercholesterolemic proprotein convertase subtilisin/kexin-9 (PCSK9) minipigs. Fifteen hypercholesterolemic PCSK9 minipigs were randomized to percutaneous coronary intervention with Magmaris, Absorb or Desolve BRS. Optical coherence tomography (OCT) scans were performed at baseline and 6-months followed by histology. Matched OCT analysis showed minimal lumen area decreased from 7.62 ± 1.54 mm2 at baseline to 2.12 ± 0.92 mm2 at follow-up in the Magmaris group, from 6.99 ± 1.49 mm2 to 3.07 ± 1.52 mm2 in the Absorb group, and from 6.05 ± 1.11 mm2 to 2.65 ± 0.93 mm2 in the Desolve group. Histologic examination revealed advanced human-like stenosis. BRS implantation in hypercholesterolemic PCSK9 minipigs induced human-like stenoses and may serve as a feasible preclinical model for obstructive coronary artery disease.

KW - Animal model

KW - Atherosclerosis

KW - Bioresorbable stent

KW - Fibroatheroma

KW - Obstructive coronary stenosis

KW - Percutaneous coronary intervention

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U2 - 10.1007/s12265-025-10607-0

DO - 10.1007/s12265-025-10607-0

M3 - Journal article

C2 - 40131655

AN - SCOPUS:105000971326

SN - 1937-5387

JO - Journal of Cardiovascular Translational Research

JF - Journal of Cardiovascular Translational Research

ER -

Induced Human-like Coronary Stenosis in Hypercholesterolemic PCSK9 Minipigs

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