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Increased expression of TCR vbeta5.1 and 8 in mucosal T-cell lines cultured from patients with Crohn disease

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  • The Department of Hepatology and Gastroenterology V
  • The Department og Pulmunary Medicine
  • Department of Human Genetics
BACKGROUND: Characterization of the T-cell receptor variable beta chain (Vbeta) repertoire in inflamed mucosa has been used to identify disease-relevant T-cell populations and antigens in Crohn disease (CD). In vitro expansion of mucosal T cells may reveal changes in Vbeta repertoire not apparent in fresh isolates and we aimed to identify Vbeta subpopulations implicated in Crohn disease. METHODS: In vivo activated mucosal T cells were cultivated using IL-2 and IL-4 from biopsies of whole colonic mucosa without use of Vbeta-modifying exogenous antigen or feeder cells. The Vbeta gene expression in mucosal T-cell cultures was determined in 30 patients with CD and 12 healthy controls using reverse transcriptase polymerase reaction (RT-PCR) covering all 23 functional Vbeta families and the Vbeta receptor prevalence was evaluated by flow cytometry in selected cultures. RESULTS: Early T-cell cultures from both CD patients and healthy controls showed a polyclonal Vbeta gene expression that narrowed during culture, which in CD cultures led to a significant over-expression of the Vbeta5.1 (P = 0.04) and Vbeta8 gene segments (P = 0.03). Together with Vbeta6 and Vbeta18, these Vbeta chains form a pattern of staphylococcal enterotoxin type E (SEE) responsive Vbeta chains, also over-expressed in CD cultures (P = 0.02). Further in vitro stimulation of CD cultures with SEE caused expansion of Vbeta8 receptor positive cells together with a proinflammatory cytokine response. CONCLUSIONS: CD may be associated with (super)antigen-specific Vbeta subpopulations selected during long-term cultivation of mucosal biopsies from inflamed colon.
Original languageEnglish
JournalScandinavian Journal of Gastroenterology
Pages (from-to)238-45
Number of pages7
Publication statusPublished - 2004

    Research areas

  • Adolescent, Adult, Case-Control Studies, Cell Culture Techniques, Colon, Crohn Disease, Female, Gene Expression, Genes, T-Cell Receptor beta, Humans, Intestinal Mucosa, Male, Middle Aged, Receptors, Antigen, T-Cell, alpha-beta, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes

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