In vivo validation of free-space fluorescence tomography using nuclear imaging

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Anikitos Garofalakis
  • ,
  • Albertine Dubois
  • ,
  • Bertrand Kuhnast
  • ,
  • Daniel Miotto Dupont
  • Isabelle Janssens
  • ,
  • Nicolas Mackiewicz
  • ,
  • Frédéric Dollé
  • ,
  • Bertrand Tavitian
  • ,
  • Frédéric Ducongé
  • Department of Molecular Biology
  • Department of Molecular Biology
The performance of small animal photonic imaging has been considerably improved since the development of fluorescence diffuse optical tomography (fDOT), which can reconstruct fluorescent probe distribution inside tissue. However, the quantification capabilities of this new technology are still a topic of debate, especially in comparison to classical nuclear imaging techniques. Here, we present a method to in vivo calibrate the quantity and localization of a probe provided by free-space fDOT (where no plate is compressing the mouse) with positron emission tomography (PET) and x-ray computed tomography, respectively. This methodology allowed us to demonstrate a strong linear correlation (R(2)=0.95) between fDOT and PET for probe concentrations ranging from 3 nM to 1 μM in a deep-seated organ.
Original languageEnglish
JournalChinese Optics Letters
Volume35
Issue18
Pages (from-to)3024-6
Number of pages3
ISSN1671-7694
Publication statusPublished - 15 Sep 2010

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