In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique

Dandan Song; Sohaib Shujaat; Ruiting Zhao; Yan Huang; Eman Shaheen; Jeroen Van Dessel; Kaan Orhan; Greetje Van de Velde; Ruxandra Coropciuc; Ruben Pauwels; Constantinus Politis; Reinhilde Jacobs

doi:10.5624/ISD.2020.50.3.199

In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique

Dandan Song, Sohaib Shujaat, Ruiting Zhao, Yan Huang, Eman Shaheen, Jeroen Van Dessel, Kaan Orhan, Greetje Van de Velde, Ruxandra Coropciuc, Ruben Pauwels, Constantinus Politis, Reinhilde Jacobs^*

^*Corresponding author for this work

Aarhus Institute of Advanced Studies

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

4 Citations (Scopus)

Abstract

Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.

Original language	English
Journal	Imaging Science in Dentistry
Volume	50
Issue	3
Pages (from-to)	199-208
Number of pages	10
ISSN	2233-7822
DOIs	https://doi.org/10.5624/ISD.2020.50.3.199
Publication status	Published - Sept 2020

Keywords

Jaw
Radiology
Rats
Vascular remodeling

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10.5624/ISD.2020.50.3.199

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Song, D., Shujaat, S., Zhao, R., Huang, Y., Shaheen, E., Van Dessel, J., Orhan, K., Van de Velde, G., Coropciuc, R., Pauwels, R., Politis, C., & Jacobs, R. (2020). In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique. Imaging Science in Dentistry, 50(3), 199-208. https://doi.org/10.5624/ISD.2020.50.3.199

@article{5d2fe8b5326a43e9ae76ffe4227c3b32,

title = "In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique",

abstract = "Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.",

keywords = "Jaw, Radiology, Rats, Vascular remodeling",

author = "Dandan Song and Sohaib Shujaat and Ruiting Zhao and Yan Huang and Eman Shaheen and {Van Dessel}, Jeroen and Kaan Orhan and {Van de Velde}, Greetje and Ruxandra Coropciuc and Ruben Pauwels and Constantinus Politis and Reinhilde Jacobs",

year = "2020",

month = sep,

doi = "10.5624/ISD.2020.50.3.199",

language = "English",

volume = "50",

pages = "199--208",

journal = "Imaging Science in Dentistry",

issn = "2233-7822",

publisher = "Korean Academy of Oral and Maxillofacial Radiology",

number = "3",

}

Song, D, Shujaat, S, Zhao, R, Huang, Y, Shaheen, E, Van Dessel, J, Orhan, K, Van de Velde, G, Coropciuc, R, Pauwels, R, Politis, C & Jacobs, R 2020, 'In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique', Imaging Science in Dentistry, vol. 50, no. 3, pp. 199-208. https://doi.org/10.5624/ISD.2020.50.3.199

In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique. / Song, Dandan; Shujaat, Sohaib; Zhao, Ruiting et al.
In: Imaging Science in Dentistry, Vol. 50, No. 3, 09.2020, p. 199-208.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model

T2 - A novel HR-MRI and micro-CT fusion technique

AU - Song, Dandan

AU - Shujaat, Sohaib

AU - Zhao, Ruiting

AU - Huang, Yan

AU - Shaheen, Eman

AU - Van Dessel, Jeroen

AU - Orhan, Kaan

AU - Van de Velde, Greetje

AU - Coropciuc, Ruxandra

AU - Pauwels, Ruben

AU - Politis, Constantinus

AU - Jacobs, Reinhilde

PY - 2020/9

Y1 - 2020/9

N2 - Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.

AB - Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization.

KW - Jaw

KW - Radiology

KW - Rats

KW - Vascular remodeling

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U2 - 10.5624/ISD.2020.50.3.199

DO - 10.5624/ISD.2020.50.3.199

M3 - Journal article

AN - SCOPUS:85092094160

SN - 2233-7822

VL - 50

SP - 199

EP - 208

JO - Imaging Science in Dentistry

JF - Imaging Science in Dentistry

IS - 3

ER -

In vivo quantification of mandibular bone remodeling and vascular changes in a wistar rat model: A novel HR-MRI and micro-CT fusion technique

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