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In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains

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In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains. / Jensen, Brita Dahl; Knorr, Kamilla; Nicolaisen, Mogens.

In: European Journal of Plant Pathology, Vol. 146, No. 3, 11.2016, p. 657-670.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Jensen BD, Knorr K, Nicolaisen M. In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains. European Journal of Plant Pathology. 2016 Nov;146(3):657-670. doi: 10.1007/s10658-016-0950-6

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Jensen, Brita Dahl ; Knorr, Kamilla ; Nicolaisen, Mogens. / In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains. In: European Journal of Plant Pathology. 2016 ; Vol. 146, No. 3. pp. 657-670.

Bibtex

@article{2dc2351630fa45aea636226dfc1cc554,
title = "In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains",
abstract = "Competitive effects between Fusarium graminearum, causing Fusarium head blight, and the endophyte Epicoccum nigrum, were performed in in vitro competition assays between the two species. Two E. nigrum isolates were isolated from wheat grains and tested as competitors against two F. graminearum isolates. A dual petri dish assay showed that E. nigrum reduced the mycelial growth of F. graminearum and vice versa. A glass slide assay revealed that E. nigrum crude cultural filtrate also had reducing effect on the growth of F. graminearum comparable to that of E. nigrum spore suspensions. Microscopy showed hyphae of F. graminearum and E. nigrum with many side branches when in close proximity, in contrast to pronounced apical hyphal growth when growing alone. Combinations of F. graminearum and E. nigrum on sterilised wheat grains were studied over time by qPCR. F. graminearum biomass was significantly reduced in inoculations applying E. nigrum three days prior to F. graminearum. In conclusion, these results showed competition and mycelial behaviour effects between F. graminearum and E. nigrum and support that E. nigrum may have potential to reduce F. graminearum infections in wheat. Competition experiments should be carried out in planta to study the interaction further.",
keywords = "Biocontrol, Endophytes, Fungal competition, qPCR, Wheat grains",
author = "Jensen, {Brita Dahl} and Kamilla Knorr and Mogens Nicolaisen",
year = "2016",
month = nov,
doi = "10.1007/s10658-016-0950-6",
language = "English",
volume = "146",
pages = "657--670",
journal = "European Journal of Plant Pathology",
issn = "0929-1873",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains

AU - Jensen, Brita Dahl

AU - Knorr, Kamilla

AU - Nicolaisen, Mogens

PY - 2016/11

Y1 - 2016/11

N2 - Competitive effects between Fusarium graminearum, causing Fusarium head blight, and the endophyte Epicoccum nigrum, were performed in in vitro competition assays between the two species. Two E. nigrum isolates were isolated from wheat grains and tested as competitors against two F. graminearum isolates. A dual petri dish assay showed that E. nigrum reduced the mycelial growth of F. graminearum and vice versa. A glass slide assay revealed that E. nigrum crude cultural filtrate also had reducing effect on the growth of F. graminearum comparable to that of E. nigrum spore suspensions. Microscopy showed hyphae of F. graminearum and E. nigrum with many side branches when in close proximity, in contrast to pronounced apical hyphal growth when growing alone. Combinations of F. graminearum and E. nigrum on sterilised wheat grains were studied over time by qPCR. F. graminearum biomass was significantly reduced in inoculations applying E. nigrum three days prior to F. graminearum. In conclusion, these results showed competition and mycelial behaviour effects between F. graminearum and E. nigrum and support that E. nigrum may have potential to reduce F. graminearum infections in wheat. Competition experiments should be carried out in planta to study the interaction further.

AB - Competitive effects between Fusarium graminearum, causing Fusarium head blight, and the endophyte Epicoccum nigrum, were performed in in vitro competition assays between the two species. Two E. nigrum isolates were isolated from wheat grains and tested as competitors against two F. graminearum isolates. A dual petri dish assay showed that E. nigrum reduced the mycelial growth of F. graminearum and vice versa. A glass slide assay revealed that E. nigrum crude cultural filtrate also had reducing effect on the growth of F. graminearum comparable to that of E. nigrum spore suspensions. Microscopy showed hyphae of F. graminearum and E. nigrum with many side branches when in close proximity, in contrast to pronounced apical hyphal growth when growing alone. Combinations of F. graminearum and E. nigrum on sterilised wheat grains were studied over time by qPCR. F. graminearum biomass was significantly reduced in inoculations applying E. nigrum three days prior to F. graminearum. In conclusion, these results showed competition and mycelial behaviour effects between F. graminearum and E. nigrum and support that E. nigrum may have potential to reduce F. graminearum infections in wheat. Competition experiments should be carried out in planta to study the interaction further.

KW - Biocontrol

KW - Endophytes

KW - Fungal competition

KW - qPCR

KW - Wheat grains

U2 - 10.1007/s10658-016-0950-6

DO - 10.1007/s10658-016-0950-6

M3 - Journal article

VL - 146

SP - 657

EP - 670

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

IS - 3

ER -