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In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains
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In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains. / Jensen, Brita Dahl; Knorr, Kamilla; Nicolaisen, Mogens.
In: European Journal of Plant Pathology, Vol. 146, No. 3, 11.2016, p. 657-670.Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
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TY - JOUR
T1 - In vitro competition between Fusarium graminearum and Epicoccum nigrum on media and wheat grains
AU - Jensen, Brita Dahl
AU - Knorr, Kamilla
AU - Nicolaisen, Mogens
PY - 2016/11
Y1 - 2016/11
N2 - Competitive effects between Fusarium graminearum, causing Fusarium head blight, and the endophyte Epicoccum nigrum, were performed in in vitro competition assays between the two species. Two E. nigrum isolates were isolated from wheat grains and tested as competitors against two F. graminearum isolates. A dual petri dish assay showed that E. nigrum reduced the mycelial growth of F. graminearum and vice versa. A glass slide assay revealed that E. nigrum crude cultural filtrate also had reducing effect on the growth of F. graminearum comparable to that of E. nigrum spore suspensions. Microscopy showed hyphae of F. graminearum and E. nigrum with many side branches when in close proximity, in contrast to pronounced apical hyphal growth when growing alone. Combinations of F. graminearum and E. nigrum on sterilised wheat grains were studied over time by qPCR. F. graminearum biomass was significantly reduced in inoculations applying E. nigrum three days prior to F. graminearum. In conclusion, these results showed competition and mycelial behaviour effects between F. graminearum and E. nigrum and support that E. nigrum may have potential to reduce F. graminearum infections in wheat. Competition experiments should be carried out in planta to study the interaction further.
AB - Competitive effects between Fusarium graminearum, causing Fusarium head blight, and the endophyte Epicoccum nigrum, were performed in in vitro competition assays between the two species. Two E. nigrum isolates were isolated from wheat grains and tested as competitors against two F. graminearum isolates. A dual petri dish assay showed that E. nigrum reduced the mycelial growth of F. graminearum and vice versa. A glass slide assay revealed that E. nigrum crude cultural filtrate also had reducing effect on the growth of F. graminearum comparable to that of E. nigrum spore suspensions. Microscopy showed hyphae of F. graminearum and E. nigrum with many side branches when in close proximity, in contrast to pronounced apical hyphal growth when growing alone. Combinations of F. graminearum and E. nigrum on sterilised wheat grains were studied over time by qPCR. F. graminearum biomass was significantly reduced in inoculations applying E. nigrum three days prior to F. graminearum. In conclusion, these results showed competition and mycelial behaviour effects between F. graminearum and E. nigrum and support that E. nigrum may have potential to reduce F. graminearum infections in wheat. Competition experiments should be carried out in planta to study the interaction further.
KW - Biocontrol
KW - Endophytes
KW - Fungal competition
KW - qPCR
KW - Wheat grains
U2 - 10.1007/s10658-016-0950-6
DO - 10.1007/s10658-016-0950-6
M3 - Journal article
VL - 146
SP - 657
EP - 670
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
SN - 0929-1873
IS - 3
ER -