Aarhus University Seal / Aarhus Universitets segl

IN SUBJECTS ALLERGIC TO GRASS POLLEN, BASOPHIL SENSITIVITY DECREASES DURING SUBCUTANEOUS IMMUNOTHERAPY DUE TO BOTH HUMORAL FACTORS AND CELLULAR DESENSITIZATION

Research output: Contribution to conferencePosterResearchpeer-review

Standard

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@conference{6bafc51b49944d6b9100f067e157ac12,
title = "IN SUBJECTS ALLERGIC TO GRASS POLLEN, BASOPHIL SENSITIVITY DECREASES DURING SUBCUTANEOUS IMMUNOTHERAPY DUE TO BOTH HUMORAL FACTORS AND CELLULAR DESENSITIZATION",
abstract = "Johannes Martin Schmid, Ronald Dahl, and Hans Juergen HoffmannDepartment of Respiratory Medicine, Aarhus, DenmarkBackground: The major effect of SCIT is a change inallergen specific immunoglobulin. We measure the contributionof competing immunoglobulin to the effect of SCIT inpatients with allergic rhino-conjunctivitis with basophil activationtests (BAT).Methods: We have randomized 24 patients to a treatment(18) and an open control group (6). Repeated BATwere performed at baseline and througout treatment. Heparinizedblood was centrifuged, plasma was removed, thecells washed twice, then reconstituted with plasma fromthe present and baseline visit, and medium. The cells were406 ESCCA AND ICyS ABSTRACTSCytometry Part B: Clinical Cytometryincubated with log dilutions of grass pollen extract andwashed again. Basophil activation was measured as the percentageof CD63 expression on CD193{\th}basophils. TheLC50 was calculated as the log of the allergen concentrationleading to half maximum basophil activation. Specific IgEand IgG4 was measured using ISAC panel.Results: We found a 15-fold reduction in basophil sensitivityin the treated group during updosing, compared totheir baseline sensitivity (n ¼ 18; P < 0.001). The sensitivitystill decreases on maintenance dose, and is nowapproaching a 60-fold reduction. There is no change in sensitivityin the control group.We show an effect due to changes in IgX, but also thatthere appears to be a desensitization of basophils independentof IgX with an about six-fold decrease in cellular sensitivity.Conclusion: We found an increasing protective effectof SCIT after 1 year of treatment measured by basophil activationtest. During the early treatment period, this effect isprimarily based on a shift towards protecting IgX, whilethere seems to be a cellular desensitisation after 1 year.Selected for presentation during the Poster Walk on Friday.(2011), Abstracts of the 11th Euroconference on Clinical Cell Analysis. Dublin, Ireland, 14–17 September 2011. Cytometry, 80B: 390–429. doi: 10.1002/cyto.b.20619",
keywords = "basophil activation test, allergy",
author = "Schmid, {Johannes Martin} and Ronald Dahl and Hoffmann, {Hans J{\"u}rgen}",
year = "2011",
month = sep,
day = "20",
language = "English",

}

RIS

TY - CONF

T1 - IN SUBJECTS ALLERGIC TO GRASS POLLEN, BASOPHIL SENSITIVITY DECREASES DURING SUBCUTANEOUS IMMUNOTHERAPY DUE TO BOTH HUMORAL FACTORS AND CELLULAR DESENSITIZATION

AU - Schmid, Johannes Martin

AU - Dahl, Ronald

AU - Hoffmann, Hans Jürgen

PY - 2011/9/20

Y1 - 2011/9/20

N2 - Johannes Martin Schmid, Ronald Dahl, and Hans Juergen HoffmannDepartment of Respiratory Medicine, Aarhus, DenmarkBackground: The major effect of SCIT is a change inallergen specific immunoglobulin. We measure the contributionof competing immunoglobulin to the effect of SCIT inpatients with allergic rhino-conjunctivitis with basophil activationtests (BAT).Methods: We have randomized 24 patients to a treatment(18) and an open control group (6). Repeated BATwere performed at baseline and througout treatment. Heparinizedblood was centrifuged, plasma was removed, thecells washed twice, then reconstituted with plasma fromthe present and baseline visit, and medium. The cells were406 ESCCA AND ICyS ABSTRACTSCytometry Part B: Clinical Cytometryincubated with log dilutions of grass pollen extract andwashed again. Basophil activation was measured as the percentageof CD63 expression on CD193þbasophils. TheLC50 was calculated as the log of the allergen concentrationleading to half maximum basophil activation. Specific IgEand IgG4 was measured using ISAC panel.Results: We found a 15-fold reduction in basophil sensitivityin the treated group during updosing, compared totheir baseline sensitivity (n ¼ 18; P < 0.001). The sensitivitystill decreases on maintenance dose, and is nowapproaching a 60-fold reduction. There is no change in sensitivityin the control group.We show an effect due to changes in IgX, but also thatthere appears to be a desensitization of basophils independentof IgX with an about six-fold decrease in cellular sensitivity.Conclusion: We found an increasing protective effectof SCIT after 1 year of treatment measured by basophil activationtest. During the early treatment period, this effect isprimarily based on a shift towards protecting IgX, whilethere seems to be a cellular desensitisation after 1 year.Selected for presentation during the Poster Walk on Friday.(2011), Abstracts of the 11th Euroconference on Clinical Cell Analysis. Dublin, Ireland, 14–17 September 2011. Cytometry, 80B: 390–429. doi: 10.1002/cyto.b.20619

AB - Johannes Martin Schmid, Ronald Dahl, and Hans Juergen HoffmannDepartment of Respiratory Medicine, Aarhus, DenmarkBackground: The major effect of SCIT is a change inallergen specific immunoglobulin. We measure the contributionof competing immunoglobulin to the effect of SCIT inpatients with allergic rhino-conjunctivitis with basophil activationtests (BAT).Methods: We have randomized 24 patients to a treatment(18) and an open control group (6). Repeated BATwere performed at baseline and througout treatment. Heparinizedblood was centrifuged, plasma was removed, thecells washed twice, then reconstituted with plasma fromthe present and baseline visit, and medium. The cells were406 ESCCA AND ICyS ABSTRACTSCytometry Part B: Clinical Cytometryincubated with log dilutions of grass pollen extract andwashed again. Basophil activation was measured as the percentageof CD63 expression on CD193þbasophils. TheLC50 was calculated as the log of the allergen concentrationleading to half maximum basophil activation. Specific IgEand IgG4 was measured using ISAC panel.Results: We found a 15-fold reduction in basophil sensitivityin the treated group during updosing, compared totheir baseline sensitivity (n ¼ 18; P < 0.001). The sensitivitystill decreases on maintenance dose, and is nowapproaching a 60-fold reduction. There is no change in sensitivityin the control group.We show an effect due to changes in IgX, but also thatthere appears to be a desensitization of basophils independentof IgX with an about six-fold decrease in cellular sensitivity.Conclusion: We found an increasing protective effectof SCIT after 1 year of treatment measured by basophil activationtest. During the early treatment period, this effect isprimarily based on a shift towards protecting IgX, whilethere seems to be a cellular desensitisation after 1 year.Selected for presentation during the Poster Walk on Friday.(2011), Abstracts of the 11th Euroconference on Clinical Cell Analysis. Dublin, Ireland, 14–17 September 2011. Cytometry, 80B: 390–429. doi: 10.1002/cyto.b.20619

KW - basophil activation test, allergy

M3 - Poster

ER -