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Improving efficiency in stereology: a study applying the proportionator and the autodisector on virtual slides

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Improving efficiency in stereology : a study applying the proportionator and the autodisector on virtual slides. / Keller, Kresten Krarup; Andersen, Ina Trolle; Andersen, Johnnie Bremholm; Hahn, Ute; Stengaard-Pedersen, Kristian; Hauge, Ellen Margrethe; Nyengaard, Jens Randel.

In: Journal of Microscopy, Vol. 251, No. 1, 2013, p. 68-76.

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@article{a0748c399a4c4f269a7d24bdaf3147b0,
title = "Improving efficiency in stereology: a study applying the proportionator and the autodisector on virtual slides",
abstract = "Cell counting in stereology is time-consuming. The proportionator is a new stereological sampling method combining automatic image analysis and non-uniform sampling. The autodisector on virtual slides combines automatic generation of disector pairs with the use of digital images. The aim of the study was to investigate the time efficiency of the proportionator and the autodisector on virtual slides compared with traditional methods in a practical application, namely the estimation of osteoclast numbers in paws from mice with experimental arthritis and control mice. Tissue slides were scanned in a digital slide scanner and the autodisector was applied on the obtained virtual tissue slides. Every slide was partitioned into fields of view, and cells were counted in all of them. Based on the original exhaustive data set comprising 100{\%} of fields of view and covering the total section area, a proportionator sampling and a systematic, uniform random sampling were simulated. We found that the proportionator was 50{\%} to 90{\%} more time efficient than systematic, uniform random sampling. The time efficiency of the autodisector on virtual slides was 60{\%} to 100{\%} better than the disector on tissue slides. We conclude that both the proportionator and the autodisector on virtual slides may improve efficiency of cell counting in stereology.",
keywords = "Animals, Arthritis, Automation, Laboratory, Bone and Bones, Cell Count, Extremities, Imaging, Three-Dimensional, Mice, Microscopy, Optical Imaging, Osteoclasts, Time Factors",
author = "Keller, {Kresten Krarup} and Andersen, {Ina Trolle} and Andersen, {Johnnie Bremholm} and Ute Hahn and Kristian Stengaard-Pedersen and Hauge, {Ellen Margrethe} and Nyengaard, {Jens Randel}",
year = "2013",
doi = "10.1111/jmi.12044",
language = "English",
volume = "251",
pages = "68--76",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Improving efficiency in stereology

T2 - a study applying the proportionator and the autodisector on virtual slides

AU - Keller, Kresten Krarup

AU - Andersen, Ina Trolle

AU - Andersen, Johnnie Bremholm

AU - Hahn, Ute

AU - Stengaard-Pedersen, Kristian

AU - Hauge, Ellen Margrethe

AU - Nyengaard, Jens Randel

PY - 2013

Y1 - 2013

N2 - Cell counting in stereology is time-consuming. The proportionator is a new stereological sampling method combining automatic image analysis and non-uniform sampling. The autodisector on virtual slides combines automatic generation of disector pairs with the use of digital images. The aim of the study was to investigate the time efficiency of the proportionator and the autodisector on virtual slides compared with traditional methods in a practical application, namely the estimation of osteoclast numbers in paws from mice with experimental arthritis and control mice. Tissue slides were scanned in a digital slide scanner and the autodisector was applied on the obtained virtual tissue slides. Every slide was partitioned into fields of view, and cells were counted in all of them. Based on the original exhaustive data set comprising 100% of fields of view and covering the total section area, a proportionator sampling and a systematic, uniform random sampling were simulated. We found that the proportionator was 50% to 90% more time efficient than systematic, uniform random sampling. The time efficiency of the autodisector on virtual slides was 60% to 100% better than the disector on tissue slides. We conclude that both the proportionator and the autodisector on virtual slides may improve efficiency of cell counting in stereology.

AB - Cell counting in stereology is time-consuming. The proportionator is a new stereological sampling method combining automatic image analysis and non-uniform sampling. The autodisector on virtual slides combines automatic generation of disector pairs with the use of digital images. The aim of the study was to investigate the time efficiency of the proportionator and the autodisector on virtual slides compared with traditional methods in a practical application, namely the estimation of osteoclast numbers in paws from mice with experimental arthritis and control mice. Tissue slides were scanned in a digital slide scanner and the autodisector was applied on the obtained virtual tissue slides. Every slide was partitioned into fields of view, and cells were counted in all of them. Based on the original exhaustive data set comprising 100% of fields of view and covering the total section area, a proportionator sampling and a systematic, uniform random sampling were simulated. We found that the proportionator was 50% to 90% more time efficient than systematic, uniform random sampling. The time efficiency of the autodisector on virtual slides was 60% to 100% better than the disector on tissue slides. We conclude that both the proportionator and the autodisector on virtual slides may improve efficiency of cell counting in stereology.

KW - Animals

KW - Arthritis

KW - Automation, Laboratory

KW - Bone and Bones

KW - Cell Count

KW - Extremities

KW - Imaging, Three-Dimensional

KW - Mice

KW - Microscopy

KW - Optical Imaging

KW - Osteoclasts

KW - Time Factors

U2 - 10.1111/jmi.12044

DO - 10.1111/jmi.12044

M3 - Journal article

VL - 251

SP - 68

EP - 76

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - 1

ER -