Improved GFR and renal plasma perfusion following remote ischaemic conditioning in a porcine kidney transplantation model

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Improved GFR and renal plasma perfusion following remote ischaemic conditioning in a porcine kidney transplantation model. / Krogstrup, Nicoline V; Soendergaard, Peter; Secher, Niels G; Ravlo, Kristian; Keller, Anna K; Toennesen, Else; Bibby, Bo M; Moldrup, Ulla; Østraat, Ernst Øyvind; Pedersen, Michael; Jorgensen, Troels M; Leuvenink, Henri; Norregaard, Rikke; Birn, Henrik; Marcussen, Niels; Jespersen, Bente.

In: Transplant International, Vol. 25, No. 9, 2012, p. 1002-1012.

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@article{2bbb90d90a68408fa838bb8b9773d725,
title = "Improved GFR and renal plasma perfusion following remote ischaemic conditioning in a porcine kidney transplantation model",
abstract = "Delayed graft function (DGF) complicates approximately 25{\%} of kidney allografts donated after brain death (DBD). Remote ischaemic conditioning (rIC) involves brief, repetitive, ischaemia in a distant tissue in connection with ischaemia/reperfusion in the target organ. rIC has been shown to induce systemic protection against ischaemic injuries. Using a porcine kidney transplantation model with donor (63 kg) recipient (15 kg) size mismatch, we investigated the effects of recipient rIC on early renal plasma perfusion and GFR. Brain death was induced in donor pigs (n = 8) and kidneys were removed and kept in cold storage until transplantation. Nephrectomized recipient pigs were randomized to rIC (n = 8) or non-rIC (n = 8) with one kidney from the same donor in each group. rIC consisted of 4 × 5 min clamping of the abdominal aorta. GFR was significantly higher in the rIC group compared with non-rIC (7.2 ml/min vs. 3.4 ml/min; ΔGFR = 3.7 ml/min, 95{\%}-CI: 0.3-7.2 ml/min, P = 0.038). Renal plasma perfusion in both cortex and medulla measured by dynamic contrast-enhanced magnetic resonance imaging (MRI) was significantly higher over time in the rIC group compared with non-rIC. This experimental study demonstrated a positive effect of rIC on early graft perfusion and function in a large animal transplantation model.",
author = "Krogstrup, {Nicoline V} and Peter Soendergaard and Secher, {Niels G} and Kristian Ravlo and Keller, {Anna K} and Else Toennesen and Bibby, {Bo M} and Ulla Moldrup and {\O}straat, {Ernst {\O}yvind} and Michael Pedersen and Jorgensen, {Troels M} and Henri Leuvenink and Rikke Norregaard and Henrik Birn and Niels Marcussen and Bente Jespersen",
note = "{\circledC} 2012 The Authors. Transplant International {\circledC} 2012 European Society for Organ Transplantation.",
year = "2012",
doi = "10.1111/j.1432-2277.2012.01522.x",
language = "English",
volume = "25",
pages = "1002--1012",
journal = "Transplant International",
issn = "0934-0874",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "9",

}

RIS

TY - JOUR

T1 - Improved GFR and renal plasma perfusion following remote ischaemic conditioning in a porcine kidney transplantation model

AU - Krogstrup, Nicoline V

AU - Soendergaard, Peter

AU - Secher, Niels G

AU - Ravlo, Kristian

AU - Keller, Anna K

AU - Toennesen, Else

AU - Bibby, Bo M

AU - Moldrup, Ulla

AU - Østraat, Ernst Øyvind

AU - Pedersen, Michael

AU - Jorgensen, Troels M

AU - Leuvenink, Henri

AU - Norregaard, Rikke

AU - Birn, Henrik

AU - Marcussen, Niels

AU - Jespersen, Bente

N1 - © 2012 The Authors. Transplant International © 2012 European Society for Organ Transplantation.

PY - 2012

Y1 - 2012

N2 - Delayed graft function (DGF) complicates approximately 25% of kidney allografts donated after brain death (DBD). Remote ischaemic conditioning (rIC) involves brief, repetitive, ischaemia in a distant tissue in connection with ischaemia/reperfusion in the target organ. rIC has been shown to induce systemic protection against ischaemic injuries. Using a porcine kidney transplantation model with donor (63 kg) recipient (15 kg) size mismatch, we investigated the effects of recipient rIC on early renal plasma perfusion and GFR. Brain death was induced in donor pigs (n = 8) and kidneys were removed and kept in cold storage until transplantation. Nephrectomized recipient pigs were randomized to rIC (n = 8) or non-rIC (n = 8) with one kidney from the same donor in each group. rIC consisted of 4 × 5 min clamping of the abdominal aorta. GFR was significantly higher in the rIC group compared with non-rIC (7.2 ml/min vs. 3.4 ml/min; ΔGFR = 3.7 ml/min, 95%-CI: 0.3-7.2 ml/min, P = 0.038). Renal plasma perfusion in both cortex and medulla measured by dynamic contrast-enhanced magnetic resonance imaging (MRI) was significantly higher over time in the rIC group compared with non-rIC. This experimental study demonstrated a positive effect of rIC on early graft perfusion and function in a large animal transplantation model.

AB - Delayed graft function (DGF) complicates approximately 25% of kidney allografts donated after brain death (DBD). Remote ischaemic conditioning (rIC) involves brief, repetitive, ischaemia in a distant tissue in connection with ischaemia/reperfusion in the target organ. rIC has been shown to induce systemic protection against ischaemic injuries. Using a porcine kidney transplantation model with donor (63 kg) recipient (15 kg) size mismatch, we investigated the effects of recipient rIC on early renal plasma perfusion and GFR. Brain death was induced in donor pigs (n = 8) and kidneys were removed and kept in cold storage until transplantation. Nephrectomized recipient pigs were randomized to rIC (n = 8) or non-rIC (n = 8) with one kidney from the same donor in each group. rIC consisted of 4 × 5 min clamping of the abdominal aorta. GFR was significantly higher in the rIC group compared with non-rIC (7.2 ml/min vs. 3.4 ml/min; ΔGFR = 3.7 ml/min, 95%-CI: 0.3-7.2 ml/min, P = 0.038). Renal plasma perfusion in both cortex and medulla measured by dynamic contrast-enhanced magnetic resonance imaging (MRI) was significantly higher over time in the rIC group compared with non-rIC. This experimental study demonstrated a positive effect of rIC on early graft perfusion and function in a large animal transplantation model.

U2 - 10.1111/j.1432-2277.2012.01522.x

DO - 10.1111/j.1432-2277.2012.01522.x

M3 - Journal article

VL - 25

SP - 1002

EP - 1012

JO - Transplant International

JF - Transplant International

SN - 0934-0874

IS - 9

ER -