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Improved enzymatic production of phenolated glycerides through alkyl phenolate intermediate

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  • Zhiyong Yang, Denmark
  • Vivian Feddern, School of Chemistry and Food, Laboratory of Food Science, University of Rio Grande, Brazil
  • Marianne Glasius
  • Zheng Guo
  • Xuebing Xu, Denmark
  • Department of Molecular Biology
  • Interdisciplinary Nanoscience Center
  • Department of Chemistry
This work reported a novel approach for synthesis of dihydrocaffoylated glycerides, consisting of 2 steps: enzymatic synthesis of octyl dihydrocaffeate (as a synthetic intermediate) from octanol and dihydrocaffeic acid (DHCA), and enzymatic interesterification of triglycerides with octyl dihydrocaffeate. Due to the good compatibility of the intermediate with triglycerides, an improved volumetric productivity (147 M h -1 (kg Novozym 435) -1) and high enzyme specific activity (up to 9.57 µmol -1min -1(g Novozym 435) -1) have been obtained.
Original languageEnglish
JournalBiotechnology Letters
Pages (from-to)673-679
Number of pages6
Publication statusPublished - 2011

    Research areas

  • Alkyl phenolate • Dihydrocaffeic acid (DHCA) • Lipase • Phenolic acids • Interesterification

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