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Implementation of a manual for working with wobbler mice and criteria for discontinuation of the experiment

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  • Bastian Ott, Institute of Anatomy, Dept. Cytology, Ruhr-University Bochum, Germany, Germany
  • Carolin Dahlke, Institute of Anatomy, Dept. Cytology, Ruhr-University Bochum, Germany, Germany
  • Karl Meller, Institute of Anatomy, Dept. Cytology, Ruhr-University Bochum, Germany, Germany
  • Markus Napirei, Institute of Anatomy, Dept. Cytology, Ruhr-University Bochum, Germany, Germany
  • Thomas Schmitt-John
  • ,
  • Beate Brand-Saberi, Institute of Anatomy, Ruhr-University Bochum, Germany, Germany
  • Carsten Theiss, Institute of Anatomy, Dept. Cytology, Ruhr-University Bochum, Germany, Germany
  • Darius Saberi, Institute of Anatomy, Dept. Cytology, Ruhr-University Bochum, Germany, Germany
Mouse breeding is of importance to a whole range of medical and biological research. There are many known mouse models for motor neuron diseases. However, it must be kept in mind that especially mouse models for amyotrophic lateral sclerosis develop severe symptoms causing intense stress. This article is designed to summarize conscientious work with the wobbler mouse, a model for the sporadic form of amyotrophic lateral sclerosis. This mouse model is characterized by a degeneration of α-motor-neurons leading to head tremor, loss of body weight and rapidly progressive paralysis. Although this mouse model has been known since 1956, there are no guidelines for breeding wobbler mice. Due to the lack of such guidelines the present study tries to close this gap and implements a manual for further studies. It includes the whole workflow in regard to wobbler mice from breeding and animal care taking, genotyping and phenotype analysis, but also gives some examples for the use of various neuronal tissues for histological investigation. Beside the progress in research a second aim should always be the enhancement of mouse welfare and reduction of stress for the laboratory animals.

Keywords
Original languageEnglish
JournalAnnals of Anatomy
Volume200
Pages (from-to)118-124
Number of pages7
ISSN0940-9602
DOIs
Publication statusPublished - Jul 2015

    Research areas

  • Amyotrophic lateral sclerosis, Wobbler

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