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Immune responses upon experimental Erysipelothrix rhusiopathiae infection of naïve and vaccinated chickens

Research output: Contribution to conferencePosterResearchpeer-review

  • Eva Wattrang, National Veterinary Institute, Uppsala, Sweden
  • ,
  • Helena Eriksson, National Veterinary Institute, Uppsala, Sweden, Sweden
  • Tomas Jinnerot, National Veterinary Institute, Uppsala, Sweden
  • Maria Perssson, National Veterinary Institute, Uppsala, Sweden, Sweden
  • Robert Söderlund, National Veterinary Institute, Uppsala, Sweden
  • ,
  • Lene Rosborg Dal
  • Mohammad Naghizadeh
  • ,
  • Tina Sørensen Dalgaard
The study aimed to monitor immune responses during Erysipelothrix rhusiopathiae (ER)
infection of chickens. The experiment comprised 39 SPF-raised female layer chickens in 3
groups (n=13). Chickens in group 1 remained uninfected. Chickens in group 3 were
vaccinated with a commercial inactivated ER vaccine at 17 days of age (day -13). Chickens in
groups 2 and 3 were inoculated with 0.5 x 1010 CFU ER at 30 days of age (day 0). Blood
samples were collected at vaccination, prior to infection (day -3) and at days 1, 3, 5, 8, 11 and
15 after infection.
On days 2-4 one chicken in group 2 showed transient moderate signs of disease. By culture,
ER was detected in blood from chickens in group 2 on day 1 (1 of 7) and on day 3 (6 of 6) at
1x102 – 1x106 CFU/ml and in group 2 on day 3 (1 of 6) at 30 CFU/ml. Among circulating
leukocytes, heterophil numbers increased prominently on day 1 for chickens in groups 2 and
3. For group 2 heterophil numbers remained elevated until day 5 and then decreased to preinfection
levels For group 3 heterophil numbers returned to pre-infection levels on day 3.
Serum MBL levels were significantly increased for chickens in group 2 and 3 on day 1. For
group 2 MBL levels increased further on day 3 and remained elevated on day 5 and returned
to pre-infection levels on day 8. For group 3 MBL levels returned to pre-infection levels on
day 3. Chickens in group 2 seroconverted to ER on day 5-8 and ER antibody levels subsequently increased. Chickens in group 3 were seropositive to ER prior to infection and
ER antibody levels were further increased after infection.
Thus, the infection elicited prompt heterophil and MBL responses and was effectively cleared
by all chickens. Vaccinated chickens did however show lower levels of bacteraemia and their
acute immune responses were of shorter duration. It is likely that the vaccine raised antibodies
to ER aided this rapid elimination of bacteria e.g. through opsonisation for phagocytic
clearance.
Original languageEnglish
Publication year2018
Publication statusPublished - 2018
Event15th Avian Immunology Research group meeting OXFORD, UK 5-7 September 2018 -
Duration: 5 Sep 20187 Sep 2018

Conference

Conference15th Avian Immunology Research group meeting OXFORD, UK 5-7 September 2018
Period05/09/201807/09/2018

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