Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells

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Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells. / Zor, Kinga; Mark, Christina; Heiskanen, Arto; Madsen, Claus Krogh; Dufva, Martin; Emnéus, Jenny; Brinch-Pedersen, Henrik; Finnie, Christine.

In: Plant Physiology and Biochemistry, Vol. 118, 09.2017, p. 71-76.

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Zor, K., Mark, C., Heiskanen, A., Madsen, C. K., Dufva, M., Emnéus, J., ... Finnie, C. (2017). Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells. Plant Physiology and Biochemistry, 118, 71-76. https://doi.org/10.1016/j.plaphy.2017.06.008

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Zor, Kinga ; Mark, Christina ; Heiskanen, Arto ; Madsen, Claus Krogh ; Dufva, Martin ; Emnéus, Jenny ; Brinch-Pedersen, Henrik ; Finnie, Christine. / Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells. In: Plant Physiology and Biochemistry. 2017 ; Vol. 118. pp. 71-76.

Bibtex

@article{41e18febf5ab433da07dfe59045f13ed,
title = "Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells",
abstract = "The barley aleurone layer is an established model system for studying phytohormone signalling, enzyme secretion and programmed cell death during seed germination. Most analyses performed on the aleurone layer are end-point assays based on cell extracts, meaning each sample is only analysed at a single time point. By immobilising barley aleurone layer tissue on polydimethylsiloxane pillars in the lid of a multiwell plate, continuous monitoring of living tissue is enabled using multiple non-destructive assays in parallel. Cell viability and menadione reducing capacity were monitored in the same aleurone layer samples over time, in the presence or absence of plant hormones and other effectors. The system is also amenable to transient gene expression by particle bombardment, with simultaneous monitoring of cell death. In conclusion, the easy to handle and efficient experimental setup developed here enables continuous monitoring of tissue samples, parallelisation of assays and single cell analysis, with potential for time course studies using any plant tissue that can be immobilised, for example leaves or epidermal peels.",
author = "Kinga Zor and Christina Mark and Arto Heiskanen and Madsen, {Claus Krogh} and Martin Dufva and Jenny Emn{\'e}us and Henrik Brinch-Pedersen and Christine Finnie",
year = "2017",
month = "9",
doi = "10.1016/j.plaphy.2017.06.008",
language = "English",
volume = "118",
pages = "71--76",
journal = "Plant Physiology and Biochemistry",
issn = "0981-9428",
publisher = "Elsevier Masson",

}

RIS

TY - JOUR

T1 - Immobilisation of barley aleurone layers enables parallelisation of assays and analysis of transient gene expression in single cells

AU - Zor, Kinga

AU - Mark, Christina

AU - Heiskanen, Arto

AU - Madsen, Claus Krogh

AU - Dufva, Martin

AU - Emnéus, Jenny

AU - Brinch-Pedersen, Henrik

AU - Finnie, Christine

PY - 2017/9

Y1 - 2017/9

N2 - The barley aleurone layer is an established model system for studying phytohormone signalling, enzyme secretion and programmed cell death during seed germination. Most analyses performed on the aleurone layer are end-point assays based on cell extracts, meaning each sample is only analysed at a single time point. By immobilising barley aleurone layer tissue on polydimethylsiloxane pillars in the lid of a multiwell plate, continuous monitoring of living tissue is enabled using multiple non-destructive assays in parallel. Cell viability and menadione reducing capacity were monitored in the same aleurone layer samples over time, in the presence or absence of plant hormones and other effectors. The system is also amenable to transient gene expression by particle bombardment, with simultaneous monitoring of cell death. In conclusion, the easy to handle and efficient experimental setup developed here enables continuous monitoring of tissue samples, parallelisation of assays and single cell analysis, with potential for time course studies using any plant tissue that can be immobilised, for example leaves or epidermal peels.

AB - The barley aleurone layer is an established model system for studying phytohormone signalling, enzyme secretion and programmed cell death during seed germination. Most analyses performed on the aleurone layer are end-point assays based on cell extracts, meaning each sample is only analysed at a single time point. By immobilising barley aleurone layer tissue on polydimethylsiloxane pillars in the lid of a multiwell plate, continuous monitoring of living tissue is enabled using multiple non-destructive assays in parallel. Cell viability and menadione reducing capacity were monitored in the same aleurone layer samples over time, in the presence or absence of plant hormones and other effectors. The system is also amenable to transient gene expression by particle bombardment, with simultaneous monitoring of cell death. In conclusion, the easy to handle and efficient experimental setup developed here enables continuous monitoring of tissue samples, parallelisation of assays and single cell analysis, with potential for time course studies using any plant tissue that can be immobilised, for example leaves or epidermal peels.

U2 - 10.1016/j.plaphy.2017.06.008

DO - 10.1016/j.plaphy.2017.06.008

M3 - Journal article

VL - 118

SP - 71

EP - 76

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

ER -