Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins

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Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins. / Mortensen, Michael Rosholm; Nielsen, Nanna Louise; Palmfeldt, Johan; Gothelf, Kurt Vesterager.

In: Organic and Biomolecular Chemistry, Vol. 17, No. 6, 01.01.2019, p. 1379-1383.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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MLA

Mortensen, Michael Rosholm et al. "Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins". Organic and Biomolecular Chemistry. 2019, 17(6). 1379-1383. https://doi.org/10.1039/C8OB03017K

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Mortensen, Michael Rosholm ; Nielsen, Nanna Louise ; Palmfeldt, Johan ; Gothelf, Kurt Vesterager. / Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins. In: Organic and Biomolecular Chemistry. 2019 ; Vol. 17, No. 6. pp. 1379-1383.

Bibtex

@article{b972b50cca144800ba4cb095f5d24a07,
title = "Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins",
abstract = "Probes for affinity guided conjugation have shown great promise for the preparation of high-quality protein conjugates. However, such probes are often limited in their protein scope. Here, we demonstrate the synthesis and use of imidazole carbamate probes for affinity guided conjugation of an azide handle to a wide variety of metal binding proteins such as antibodies, enzymes, nanobodies and His6-tagged proteins. The azide handles were used to link dyes to the proteins for gel analysis and binding studies. In a comparative study we find that the probe containing three NTA groups show higher selectivity than the probe containing two. In a mixture of proteins, the probe shows preference for labelling His6-tagged proteins.",
author = "Mortensen, {Michael Rosholm} and Nielsen, {Nanna Louise} and Johan Palmfeldt and Gothelf, {Kurt Vesterager}",
year = "2019",
month = "1",
day = "1",
doi = "10.1039/C8OB03017K",
language = "English",
volume = "17",
pages = "1379--1383",
journal = "Organic & Biomolecular Chemistry",
issn = "1477-0520",
publisher = "ROYAL SOC CHEMISTRY",
number = "6",

}

RIS

TY - JOUR

T1 - Imidazole carbamate probes for affinity guided azide-transfer to metal-binding proteins

AU - Mortensen, Michael Rosholm

AU - Nielsen, Nanna Louise

AU - Palmfeldt, Johan

AU - Gothelf, Kurt Vesterager

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Probes for affinity guided conjugation have shown great promise for the preparation of high-quality protein conjugates. However, such probes are often limited in their protein scope. Here, we demonstrate the synthesis and use of imidazole carbamate probes for affinity guided conjugation of an azide handle to a wide variety of metal binding proteins such as antibodies, enzymes, nanobodies and His6-tagged proteins. The azide handles were used to link dyes to the proteins for gel analysis and binding studies. In a comparative study we find that the probe containing three NTA groups show higher selectivity than the probe containing two. In a mixture of proteins, the probe shows preference for labelling His6-tagged proteins.

AB - Probes for affinity guided conjugation have shown great promise for the preparation of high-quality protein conjugates. However, such probes are often limited in their protein scope. Here, we demonstrate the synthesis and use of imidazole carbamate probes for affinity guided conjugation of an azide handle to a wide variety of metal binding proteins such as antibodies, enzymes, nanobodies and His6-tagged proteins. The azide handles were used to link dyes to the proteins for gel analysis and binding studies. In a comparative study we find that the probe containing three NTA groups show higher selectivity than the probe containing two. In a mixture of proteins, the probe shows preference for labelling His6-tagged proteins.

UR - http://www.scopus.com/inward/record.url?scp=85061119617&partnerID=8YFLogxK

U2 - 10.1039/C8OB03017K

DO - 10.1039/C8OB03017K

M3 - Journal article

VL - 17

SP - 1379

EP - 1383

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

SN - 1477-0520

IS - 6

ER -