Lotus japonicus Nuclear Factor YA1, a nodule emergence stage-specific regulator of auxin signalling

TY - JOUR

T1 - Lotus japonicus Nuclear Factor YA1, a nodule emergence stage-specific regulator of auxin signalling

AU - Shrestha, Arina

AU - Zhong, Sihui

AU - Therrien, Jasmine

AU - Huebert, Terry

AU - Sato, Shusei

AU - Mun, Terry

AU - Andersen, Stig U

AU - Stougaard, Jens

AU - Lepage, Agnes

AU - Niebel, Andreas

AU - Ross, Loretta

AU - Szczyglowski, Krzysztof

N1 - This article is protected by copyright. All rights reserved.

PY - 2021/2

Y1 - 2021/2

N2 - Organogenesis of legume root nodules begins with the nodulation factor-dependent stimulation of compatible root cells to initiate divisions, signifying an early nodule primordium formation event. This is followed by cellular differentiation, including cell expansion and vascular bundle formation, and we previously showed that Lotus japonicus NF-YA1 is essential for this process, presumably by regulating three members of the SHORT INTERNODES/STYLISH (STY) transcription factor gene family. In this study, we used combined genetics, genomics and cell biology approaches to characterize the role of STY genes during root nodule formation and to test a hypothesis that they mediate nodule development by stimulating auxin signalling. We show here that L. japonicus STYs are required for nodule emergence. This is attributed to the NF-YA1-dependent regulatory cascade, comprising STY genes and their downstream targets, YUCCA1 and YUCCA11, involved in a local auxin biosynthesis at the post-initial cell division stage. An analogous NF-YA1/STY regulatory module seems to operate in Medicago truncatula in association with the indeterminate nodule patterning. Our data define L. japonicus and M. truncatula NF-YA1 genes as important nodule emergence stage-specific regulators of auxin signalling while indicating that the inductive stage and subsequent formation of early nodule primordia are mediated through an independent mechanism(s).

AB - Organogenesis of legume root nodules begins with the nodulation factor-dependent stimulation of compatible root cells to initiate divisions, signifying an early nodule primordium formation event. This is followed by cellular differentiation, including cell expansion and vascular bundle formation, and we previously showed that Lotus japonicus NF-YA1 is essential for this process, presumably by regulating three members of the SHORT INTERNODES/STYLISH (STY) transcription factor gene family. In this study, we used combined genetics, genomics and cell biology approaches to characterize the role of STY genes during root nodule formation and to test a hypothesis that they mediate nodule development by stimulating auxin signalling. We show here that L. japonicus STYs are required for nodule emergence. This is attributed to the NF-YA1-dependent regulatory cascade, comprising STY genes and their downstream targets, YUCCA1 and YUCCA11, involved in a local auxin biosynthesis at the post-initial cell division stage. An analogous NF-YA1/STY regulatory module seems to operate in Medicago truncatula in association with the indeterminate nodule patterning. Our data define L. japonicus and M. truncatula NF-YA1 genes as important nodule emergence stage-specific regulators of auxin signalling while indicating that the inductive stage and subsequent formation of early nodule primordia are mediated through an independent mechanism(s).

KW - ARABIDOPSIS

KW - BINDING TRANSCRIPTION FACTORS

KW - BIOSYNTHESIS

KW - FAMILY GENES

KW - KINASE GENE

KW - LATERAL ROOT DEVELOPMENT

KW - LOTUS-JAPONICUS

KW - Lotus

KW - Medicago

KW - NF&#8208

KW - ORGANOGENESIS

KW - PLANT

KW - RHIZOBIAL INFECTION

KW - YA1

KW - auxin

KW - legume

KW - root nodule

KW - symbiosis

UR - http://www.scopus.com/inward/record.url?scp=85096786685&partnerID=8YFLogxK

U2 - 10.1111/nph.16950

DO - 10.1111/nph.16950

M3 - Journal article

C2 - 32978812

SN - 0028-646X

VL - 229

SP - 1535

EP - 1552

JO - New Phytologist

JF - New Phytologist

IS - 3

ER -