IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites

Ervinas Gaidamauskas; Claus Gyrup Nielsen; Henning Bünsow Boldt; Vivien R Schack; Michael Toft Overgaard; Lisbeth S Laursen; Claus Oxvig

IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites

Ervinas Gaidamauskas, Claus Gyrup Nielsen, Henning Bünsow Boldt, Vivien R Schack, Michael Toft Overgaard, Lisbeth S Laursen, Claus Oxvig

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

22 Citations (Scopus)

Abstract

Pregnancy-associated plasma protein-A (PAPP-A) is a local regulator of insulin-like growth factor (IGF) bioavailability in physiological systems, but many structural and functional aspects of the metzincin metalloproteinase remain to be elucidated. PAPP-A cleaves IGF binding protein (IGFBP)-4 and IGFBP-5. Cleavage of IGFBP-4, but not IGFBP-5, depends on the binding of IGF before proteolysis by PAPP-A can occur. The paralogue PAPP-A2 has two substrates among the six IGFBPs: IGFBP-3 and IGFBP-5.

Original language	English
Journal	BBA General Subjects
Volume	1830
Issue	3
Pages (from-to)	2701-2709
Number of pages	9
ISSN	0304-4165
Publication status	Published - Mar 2013

Keywords

Amino Acid Sequence
Binding Sites
Female
HEK293 Cells
Humans
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Protein 5
Models, Molecular
Molecular Sequence Data
Pregnancy
Pregnancy-Associated Plasma Protein-A
Protein Binding
Protein Interaction Domains and Motifs
Proteolysis
Recombinant Fusion Proteins
Somatomedins
Substrate Specificity
Transfection

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Cite this

Gaidamauskas, E., Nielsen, C. G., Boldt, H. B., Schack, V. R., Overgaard, M. T., Laursen, L. S., & Oxvig, C. (2013). IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites. BBA General Subjects, 1830(3), 2701-2709. http://www.sciencedirect.com/science/article/pii/S0304416512003133

@article{d4f1adb6e35d418f9ecb9da23f92071e,

title = "IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites",

abstract = "Pregnancy-associated plasma protein-A (PAPP-A) is a local regulator of insulin-like growth factor (IGF) bioavailability in physiological systems, but many structural and functional aspects of the metzincin metalloproteinase remain to be elucidated. PAPP-A cleaves IGF binding protein (IGFBP)-4 and IGFBP-5. Cleavage of IGFBP-4, but not IGFBP-5, depends on the binding of IGF before proteolysis by PAPP-A can occur. The paralogue PAPP-A2 has two substrates among the six IGFBPs: IGFBP-3 and IGFBP-5.",

keywords = "Amino Acid Sequence, Binding Sites, Female, HEK293 Cells, Humans, Insulin-Like Growth Factor Binding Protein 3, Insulin-Like Growth Factor Binding Protein 5, Models, Molecular, Molecular Sequence Data, Pregnancy, Pregnancy-Associated Plasma Protein-A, Protein Binding, Protein Interaction Domains and Motifs, Proteolysis, Recombinant Fusion Proteins, Somatomedins, Substrate Specificity, Transfection",

author = "Ervinas Gaidamauskas and Nielsen, {Claus Gyrup} and Boldt, {Henning B{\"u}nsow} and Schack, {Vivien R} and Overgaard, {Michael Toft} and Laursen, {Lisbeth S} and Claus Oxvig",

year = "2013",

month = mar,

language = "English",

volume = "1830",

pages = "2701--2709",

journal = "BBA General Subjects",

issn = "0304-4165",

publisher = "Elsevier B.V.",

number = "3",

}

Gaidamauskas, E, Nielsen, CG, Boldt, HB, Schack, VR, Overgaard, MT, Laursen, LS & Oxvig, C 2013, 'IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites', BBA General Subjects, vol. 1830, no. 3, pp. 2701-2709. <http://www.sciencedirect.com/science/article/pii/S0304416512003133>

IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites. / Gaidamauskas, Ervinas; Nielsen, Claus Gyrup; Boldt, Henning Bünsow et al.
In: BBA General Subjects, Vol. 1830, No. 3, 03.2013, p. 2701-2709.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A)

T2 - multiple PAPP-A-IGFBP interaction sites

AU - Gaidamauskas, Ervinas

AU - Nielsen, Claus Gyrup

AU - Boldt, Henning Bünsow

AU - Schack, Vivien R

AU - Overgaard, Michael Toft

AU - Laursen, Lisbeth S

AU - Oxvig, Claus

PY - 2013/3

Y1 - 2013/3

N2 - Pregnancy-associated plasma protein-A (PAPP-A) is a local regulator of insulin-like growth factor (IGF) bioavailability in physiological systems, but many structural and functional aspects of the metzincin metalloproteinase remain to be elucidated. PAPP-A cleaves IGF binding protein (IGFBP)-4 and IGFBP-5. Cleavage of IGFBP-4, but not IGFBP-5, depends on the binding of IGF before proteolysis by PAPP-A can occur. The paralogue PAPP-A2 has two substrates among the six IGFBPs: IGFBP-3 and IGFBP-5.

AB - Pregnancy-associated plasma protein-A (PAPP-A) is a local regulator of insulin-like growth factor (IGF) bioavailability in physiological systems, but many structural and functional aspects of the metzincin metalloproteinase remain to be elucidated. PAPP-A cleaves IGF binding protein (IGFBP)-4 and IGFBP-5. Cleavage of IGFBP-4, but not IGFBP-5, depends on the binding of IGF before proteolysis by PAPP-A can occur. The paralogue PAPP-A2 has two substrates among the six IGFBPs: IGFBP-3 and IGFBP-5.

KW - Amino Acid Sequence

KW - Binding Sites

KW - Female

KW - HEK293 Cells

KW - Humans

KW - Insulin-Like Growth Factor Binding Protein 3

KW - Insulin-Like Growth Factor Binding Protein 5

KW - Models, Molecular

KW - Molecular Sequence Data

KW - Pregnancy

KW - Pregnancy-Associated Plasma Protein-A

KW - Protein Binding

KW - Protein Interaction Domains and Motifs

KW - Proteolysis

KW - Recombinant Fusion Proteins

KW - Somatomedins

KW - Substrate Specificity

KW - Transfection

M3 - Journal article

C2 - 23671931

SN - 0304-4165

VL - 1830

SP - 2701

EP - 2709

JO - BBA General Subjects

JF - BBA General Subjects

IS - 3

ER -

IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): multiple PAPP-A-IGFBP interaction sites

Abstract

Keywords

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