IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning

Nicolas Taulet; Benjamin Vitre; Christelle Anguille; Audrey Douanier; Murielle Rocancourt; Michael Taschner; Esben Lorentzen; Arnaud Echard; Benedicte Delaval

doi:10.1038/s41467-017-01479-3

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning

Nicolas Taulet, Benjamin Vitre, Christelle Anguille, Audrey Douanier, Murielle Rocancourt, Michael Taschner, Esben Lorentzen, Arnaud Echard, Benedicte Delaval

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Cytokinesis mediates the physical separation of dividing cells and, in 3D epithelia, provides a spatial landmark for lumen formation. Here, we unravel an unexpected role in cytokinesis for proteins of the intraflagellar transport (IFT) machinery, initially characterized for their ciliary role and their link to polycystic kidney disease. Using 2D and 3D cultures of renal cells, we show that IFT proteins are required to correctly shape the central spindle, to control symmetric cleavage furrow ingression and to ensure central lumen positioning. Mechanistically, IFT88 directly interacts with the kinesin MKLP2 and is essential for the correct relocalization of the Aurora B/MKLP2 complex to the central spindle. IFT88 is thus required for proper centralspindlin distribution and central spindle microtubule organization. Overall, this work unravels a novel non-ciliary mechanism for IFT proteins at the central spindle, which could contribute to kidney cyst formation by affecting lumen positioning.

Original language	English
Article number	1928
Journal	Nature Communications
Volume	8
Issue	1
Number of pages	12
ISSN	2041-1723
DOIs	https://doi.org/10.1038/s41467-017-01479-3
Publication status	Published - 4 Dec 2017

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title = "IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning",

abstract = "Cytokinesis mediates the physical separation of dividing cells and, in 3D epithelia, provides a spatial landmark for lumen formation. Here, we unravel an unexpected role in cytokinesis for proteins of the intraflagellar transport (IFT) machinery, initially characterized for their ciliary role and their link to polycystic kidney disease. Using 2D and 3D cultures of renal cells, we show that IFT proteins are required to correctly shape the central spindle, to control symmetric cleavage furrow ingression and to ensure central lumen positioning. Mechanistically, IFT88 directly interacts with the kinesin MKLP2 and is essential for the correct relocalization of the Aurora B/MKLP2 complex to the central spindle. IFT88 is thus required for proper centralspindlin distribution and central spindle microtubule organization. Overall, this work unravels a novel non-ciliary mechanism for IFT proteins at the central spindle, which could contribute to kidney cyst formation by affecting lumen positioning.",

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IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. / Taulet, Nicolas; Vitre, Benjamin; Anguille, Christelle et al.
In: Nature Communications, Vol. 8, No. 1, 1928, 04.12.2017.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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AU - Taulet, Nicolas

AU - Vitre, Benjamin

AU - Anguille, Christelle

AU - Douanier, Audrey

AU - Rocancourt, Murielle

AU - Taschner, Michael

AU - Lorentzen, Esben

AU - Echard, Arnaud

AU - Delaval, Benedicte

PY - 2017/12/4

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AB - Cytokinesis mediates the physical separation of dividing cells and, in 3D epithelia, provides a spatial landmark for lumen formation. Here, we unravel an unexpected role in cytokinesis for proteins of the intraflagellar transport (IFT) machinery, initially characterized for their ciliary role and their link to polycystic kidney disease. Using 2D and 3D cultures of renal cells, we show that IFT proteins are required to correctly shape the central spindle, to control symmetric cleavage furrow ingression and to ensure central lumen positioning. Mechanistically, IFT88 directly interacts with the kinesin MKLP2 and is essential for the correct relocalization of the Aurora B/MKLP2 complex to the central spindle. IFT88 is thus required for proper centralspindlin distribution and central spindle microtubule organization. Overall, this work unravels a novel non-ciliary mechanism for IFT proteins at the central spindle, which could contribute to kidney cyst formation by affecting lumen positioning.

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IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning

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